| Literature DB >> 7707330 |
G Prevost1, P Couppie, P Prevost, S Gayet, P Petiau, B Cribier, H Monteil, Y Piemont.
Abstract
DNA hybridisation of 309 consecutive Staphylococcus aureus clinical isolates with oligonucleotide probes specific for genes encoding Panton-Valentine leucocidin (luk-PV) and gamma-haemolysin (hlg) revealed that 99% of randomly selected strains carried the hlg locus whereas only 2% harboured the luk-PV as well as the hlg loci. Only 1% of the strains did not possess either gene. In a clinical prospective study of independent S. aureus strains, 58 Panton-Valentine leucocidin (PVL)-producing isolates were shown to be responsible for primary skin infections, mainly furuncles (86%). Phage susceptibility patterns and pulsed field gel electrophoresis (PFGE) profiles of DNA were shown to be polymorphic epidemiological markers of PVL-producing strains. In eight patients with recurrent furuncles, the PVL-producing strains isolated either from furuncles or from the anterior nares were considered to be identical in each based upon phage sensitivity profiles or PFGE patterns.Entities:
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Year: 1995 PMID： 7707330 DOI： 10.1099/00222615-42-4-237
Source DB: PubMed Journal: J Med Microbiol ISSN： 0022-2615 Impact factor: 2.472