| Literature DB >> 7698656 |
T G Simcox1, L Fabian, K Kretz, V Hedden, M E Simcox.
Abstract
A new restriction endonuclease (ENase), SanDI, has been isolated from an unidentified species of Streptomyces. SanDI recognizes the 7-bp interrupted palindrome 5'-GG/GWCCC-3' (W = A or T) and cleaves double-stranded DNA after the second G in the sequence, producing 3-nt long 5' protruding ends. SanDI is a rare-cutting ENase and should therefore be useful for megabase mapping and vector constructions.Mesh:
Substances:
Year: 1995 PMID: 7698656 DOI: 10.1016/0378-1119(94)00711-z
Source DB: PubMed Journal: Gene ISSN: 0378-1119 Impact factor: 3.688