Literature DB >> 7697501

Automatic cell culture quantitation with TRAKCELL: application to cell toxicology and differentiation.

W Y Xu-van Opstal1, C Billardon, T Caillaud, S Carvajal-Gonzalez, G Colliot, J C Bisconte, W Rostene.   

Abstract

An automated system, TRAKCELL, was developed for the quantitation of cells in culture. It enabled cell counting, classification according to morphological cell characteristics and measurement of cell proliferation and differentiation. The system was tested on the toxic effect of ascorbic acid on rat brain catecholaminergic neurons in primary culture. In parallel, the effects of nerve growth factor, dexamethasone and forskolin on cell differentiation were studied using rat pheochromocytoma PC12 cells. The results show that the system permits rapid and reproducible measurements of cell density and of the morphological changes observed following various drug treatments.

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Year:  1994        PMID： 7697501     DOI： 10.1007/bf00755787

Source DB:  PubMed          Journal:  Cell Biol Toxicol        ISSN： 0742-2091            Impact factor:   6.691


  13 in total

1.  Automated image analysis for counting unstained cultured neurones.

Authors:  J D Clements; J M Buzy
Journal:  J Neurosci Methods       Date:  1991-01       Impact factor: 2.390

2.  An automated method for autoradiographic analysis of cultured Schwann cells.

Authors:  R R Baichwal; L Yan; A Bosler; G H DeVries
Journal:  J Neurosci Methods       Date:  1987-08       Impact factor: 2.390

3.  Study of living single cells in culture: automated recognition of cell behavior.

Authors:  P Bodin; S Papin; C Meyer; P Travo
Journal:  Lab Invest       Date:  1988-07       Impact factor: 5.662

Review 4.  Nerve growth factor-induced increase in electrical excitability and acetylcholine sensitivity of a rat pheochromocytoma cell line.

Authors:  M A Dichter; A S Tischler; L A Greene
Journal:  Nature       Date:  1977-08-11       Impact factor: 49.962

5.  Ascorbic acid in fetal rat brain.

Authors:  C C Kratzing; J D Kelly; J E Kratzing
Journal:  J Neurochem       Date:  1985-05       Impact factor: 5.372

6.  Automated image analyzing system for the quantitative study of living cells in culture.

Authors:  W Y Xu-van Opstal; C Ranger; O Lejeune; P Forgez; H Boudin; J C Bisconte; W Rostene
Journal:  Microsc Res Tech       Date:  1994-08-01       Impact factor: 2.769

7.  The use of an image analyser in human tumour clonogenic assays.

Authors:  E Kahn; J Benard; R Di Paola
Journal:  Cytometry       Date:  1986-07

8.  Treatment of PC12 cells by nerve growth factor, dexamethasone, and forskolin. Effects on cell morphology and expression of neurotensin and tyrosine hydroxylase.

Authors:  T Caillaud; W Y Opstal; V Scarceriaux; C Billardon; W Rostene
Journal:  Mol Neurobiol       Date:  1995 Apr-Jun       Impact factor: 5.590

9.  Mesencephalic dopaminergic neurons in primary cultures express functional neurotensin receptors.

Authors:  A Brouard; D Pelaprat; C Dana; M Vial; A M Lhiaubet; W Rostène
Journal:  J Neurosci       Date:  1992-04       Impact factor: 6.167

10.  Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor.

Authors:  L A Greene; A S Tischler
Journal:  Proc Natl Acad Sci U S A       Date:  1976-07       Impact factor: 11.205
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  1 in total

1.  Migration of isogenic cell lines quantified by dynamic multivariate analysis of single-cell motility.

Authors:  Mark P Harris; Eric Kim; Brandy Weidow; John P Wikswo; Vito Quaranta
Journal:  Cell Adh Migr       Date:  2008 Apr-May       Impact factor: 3.405
  1 in total

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