Literature DB >> 7695123

A PCR-based sex-determination assay in cattle based on the bovine amelogenin locus.

S Ennis1, T F Gallagher.   

Abstract

A method for determining the sex of bovine embryos has been established. Primers for a portion of the bovine amelogenin locus (AMX/Y) were used to amplify DNA present in either 0.1 microliter of blood or biopsies taken from 6-7-day-old embryos. The primers amplify a 280 bp band in females and a 280 and 217 bp bands in males. The method is rapid, does not require prior purification of DNA and contains an internal control which detects PCR failure.

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Year:  1994        PMID： 7695123     DOI： 10.1111/j.1365-2052.1994.tb00533.x

Source DB:  PubMed          Journal:  Anim Genet        ISSN： 0268-9146            Impact factor:   3.169


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