Literature DB >> 768986

Escherichia coli thioredoxin: a subunit of bacteriophage T7 DNA polymerase.

D F Mark, C C Richardson.   

Abstract

T7 DNA polymerase (DNA nucleotidyltransferase; deoxynucleosidetriphosphate:DNA deoxynucleotidyltransferase, EC 2.7.7.7) is composed of an 84,000 dalton protein specified by the gene 5 of the phage and a 12,000 dalton protein (TsnC protein) specified by the tsnC gene of E. coli [Modrich, P. & Richardson, C. C. (1975) J. Biol. Chem. 250 5515-5522]. Both proteins are necessary for T7 DNA polymerase activity and for the replication of T7 DNA. The TsnC protein is identical to thioredoxin of E. coli by the following criteria: (1) Homogeneous preparations of both proteins have TsnC and thioredoxin activity. (2) Both proteins show similar stability to heat. (3) They have identical mobilities, corresponding to a molecular weight of 12,000, on polyacrylamide gels containing sodium dodecyl sulfate. (4) Their amino-acid compositions are indistinguishabe. (5) Antibody prepared against thioredoxin inhibits TsnC activity. (6) TsnC protein isolated from purified T7 DNA polymerase has thioredoxin activity. In addition, preparations of T7 DNA polymerase itself exhibit thioredoxin activity and are partially inhibited by antibody to thioredoxin.

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Year:  1976        PMID： 768986      PMCID： PMC336002          DOI： 10.1073/pnas.73.3.780

Source DB:  PubMed          Journal:  Proc Natl Acad Sci U S A        ISSN： 0027-8424            Impact factor:   11.205


  22 in total

1.  ENZYMATIC SYNTHESIS OF DEOXYRIBONUCLEOTIDES. IV. ISOLATION AND CHARACTERIZATION OF THIOREDOXIN, THE HYDROGEN DONOR FROM ESCHERICHIA COLI B.

Authors:  T C LAURENT; E C MOORE; P REICHARD
Journal:  J Biol Chem       Date:  1964-10       Impact factor: 5.157

2.  Bacterial proteins required for replication of phage Q ribonucleic acid. Pruification and properties of host factor I, a ribonucleic acid-binding protein.

Authors:  M T Franze de Fernandez; W S Hayward; J T August
Journal:  J Biol Chem       Date:  1972-02-10       Impact factor: 5.157

3.  Control of pyrimidine biosynthesis by phage T4. II. In vitro complementation between ribonucleotide reductase mutants.

Authors:  Y C Yeh; I Tessman
Journal:  Virology       Date:  1972-03       Impact factor: 3.616

4.  Evidence for a complex regulating the in vivo activities of early enzymes induced by bacteriophage T4.

Authors:  P K Tomich; C S Chiu; M G Wovcha; G R Greenberg
Journal:  J Biol Chem       Date:  1974-12-10       Impact factor: 5.157

5.  DNA polymerases I and II in regenerating rat liver and Morris hepatomas.

Authors:  E F Baril; M D Jenkins; O E Brown; J Laszlo; H P Morris
Journal:  Cancer Res       Date:  1973-06       Impact factor: 12.701

6.  Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.

Authors:  G Fairbanks; T L Steck; D F Wallach
Journal:  Biochemistry       Date:  1971-06-22       Impact factor: 3.162

7.  A thioredoxin induced by bacteriophage T4. II. Purification and characterization.

Authors:  O Berglund; B M Sjöberg
Journal:  J Biol Chem       Date:  1970-11-25       Impact factor: 5.157

8.  Immunochemistry of thioredoxin. I. Preparation and cross-reactivity of antibodies against thioredoxin from Escherichia coli and bacteriophage T4.

Authors:  A Holmgren; B M Sjöberg
Journal:  J Biol Chem       Date:  1972-07-10       Impact factor: 5.157

9.  A DNA-unwinding protein isolated from Escherichia coli: its interaction with DNA and with DNA polymerases.

Authors:  N Sigal; H Delius; T Kornberg; M L Gefter; B Alberts
Journal:  Proc Natl Acad Sci U S A       Date:  1972-12       Impact factor: 11.205

10.  Isolation and characterization of prototrophic mutants of Escherichia coli unable to support the intracellular growth of T7.

Authors:  M Chamberlin
Journal:  J Virol       Date:  1974-09       Impact factor: 5.103
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  83 in total

1.  Overexpression of thioredoxin h leads to enhanced activity of starch debranching enzyme (pullulanase) in barley grain.

Authors:  M J Cho; J H Wong; C Marx; W Jiang; P G Lemaux; B B Buchanan
Journal:  Proc Natl Acad Sci U S A       Date:  1999-12-07       Impact factor: 11.205

2.  trans-acting mutations in loci other than kdpDE that affect kdp operon regulation in Escherichia coli: effects of cytoplasmic thiol oxidation status and nucleoid protein H-NS on kdp expression.

Authors:  A A Sardesai; J Gowrishankar
Journal:  J Bacteriol       Date:  2001-01       Impact factor: 3.490

3.  A unique loop in the DNA-binding crevice of bacteriophage T7 DNA polymerase influences primer utilization.

Authors:  K Chowdhury; S Tabor; C C Richardson
Journal:  Proc Natl Acad Sci U S A       Date:  2000-11-07       Impact factor: 11.205

4.  Isolation of an Escherichia coli mutant deficient in thioredoxin reductase.

Authors:  J Fuchs
Journal:  J Bacteriol       Date:  1977-02       Impact factor: 3.490

5.  The genome sequence of Yersinia pestis bacteriophage phiA1122 reveals an intimate history with the coliphage T3 and T7 genomes.

Authors:  Emilio Garcia; Jeffrey M Elliott; Erlan Ramanculov; Patrick S G Chain; May C Chu; Ian J Molineux
Journal:  J Bacteriol       Date:  2003-09       Impact factor: 3.490

6.  Molybdenum cofactor requirement for biotin sulfoxide reduction in Escherichia coli.

Authors:  A del Campillo-Campbell; A Campbell
Journal:  J Bacteriol       Date:  1982-02       Impact factor: 3.490

7.  Thioredoxin, glutaredoxin, and thioredoxin reductase from cultured HeLa cells.

Authors:  M L Tsang; J A Weatherbee
Journal:  Proc Natl Acad Sci U S A       Date:  1981-12       Impact factor: 11.205

8.  Replication of duplex DNA by bacteriophage T7 DNA polymerase and gene 4 protein is accompanied by hydrolysis of nucleoside 5'-triphosphates.

Authors:  R Kolodner; C C Richardson
Journal:  Proc Natl Acad Sci U S A       Date:  1977-04       Impact factor: 11.205

9.  Thioredoxin system of the photosynthetic anaerobe Chromatium vinosum.

Authors:  T C Johnson; N A Crawford; B B Buchanan
Journal:  J Bacteriol       Date:  1984-06       Impact factor: 3.490

10.  A novel rho promoter::Tn10 mutation suppresses and ftsQ1(Ts) missense mutation in an essential Escherichia coli cell division gene by a mechanism not involving polarity suppression.

Authors:  D R Storts; A Markovitz
Journal:  J Bacteriol       Date:  1991-01       Impact factor: 3.490
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