Literature DB >> 7689836

Characterization of murine E-selectin expression in vitro using novel anti-mouse E-selectin monoclonal antibodies.

C R Norton1, J M Rumberger, D K Burns, B A Wolitzky.   

Abstract

A recombinant phage containing the structural exons for mouse E-selectin has been isolated and characterized. Utilizing PCR techniques the lectin and egf domains were fused to form an artificial cDNA for expression in eukaryotic cells. Transient expression in COS cells demonstrated the lectin and egf domains were sufficient to mediate the binding of mouse and human neutrophils as well as HL60 cells. Recombinant soluble mouse E-selectin was purified and used to immunize rats to generate mAbs specific to mouse E-selectin. A panel of mAbs directed against mouse E-selectin was characterized including five that inhibit the adhesion of HL60 cells or mouse neutrophils to COS cells expressing the mouse lectin/egf domains. These mAbs have been used to characterize the expression and function of E-selectin on cytokine stimulated eEnd.2 murine endothelial cells.

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Year:  1993        PMID： 7689836     DOI： 10.1006/bbrc.1993.2037

Source DB:  PubMed          Journal:  Biochem Biophys Res Commun        ISSN： 0006-291X            Impact factor:   3.575


  12 in total

1.  Anti-inflammatory drugs and endothelial cell adhesion molecule expression in murine vascular beds.

Authors:  N Mori; Y Horie; M E Gerritsen; D C Anderson; D N Granger
Journal:  Gut       Date:  1999-02       Impact factor: 23.059

2.  Pathogenesis of skin lesions in mice with chronic proliferative dermatitis (cpdm/cpdm).

Authors:  M J Gijbels; C Zurcher; G Kraal; G R Elliott; H HogenEsch; G Schijff; H F Savelkoul; P L Bruijnzeel
Journal:  Am J Pathol       Date:  1996-03       Impact factor: 4.307

3.  Dominant role of L- and P-selectin in mediating CXC chemokine-induced neutrophil migration in vivo.

Authors:  J M Miotla; V C Ridger; P G Hellewell
Journal:  Br J Pharmacol       Date:  2001-06       Impact factor: 8.739

4.  Leukocyte adhesion in angiogenic blood vessels. Role of E-selectin, P-selectin, and beta2 integrin in lymphotoxin-mediated leukocyte recruitment in tumor microvessels.

Authors:  P Borgström; G K Hughes; P Hansell; B A Wolitsky; P Sriramarao
Journal:  J Clin Invest       Date:  1997-05-01       Impact factor: 14.808

5.  Quantitative measurement of P- and E-selectin adhesion molecules in acute pancreatitis: correlation with distant organ injury.

Authors:  A H Lundberg; D N Granger; J Russell; O Sabek; J Henry; L Gaber; M Kotb; A O Gaber
Journal:  Ann Surg       Date:  2000-02       Impact factor: 12.969

6.  L-selectin shedding regulates leukocyte recruitment.

Authors:  A Hafezi-Moghadam; K L Thomas; A J Prorock; Y Huo; K Ley
Journal:  J Exp Med       Date:  2001-04-02       Impact factor: 14.307

7.  Transit time of leukocytes rolling through venules controls cytokine-induced inflammatory cell recruitment in vivo.

Authors:  U Jung; K E Norman; K Scharffetter-Kochanek; A L Beaudet; K Ley
Journal:  J Clin Invest       Date:  1998-10-15       Impact factor: 14.808

8.  Relevance of L-selectin shedding for leukocyte rolling in vivo.

Authors:  A Hafezi-Moghadam; K Ley
Journal:  J Exp Med       Date:  1999-03-15       Impact factor: 14.307

9.  Targeted gene disruption demonstrates that P-selectin glycoprotein ligand 1 (PSGL-1) is required for P-selectin-mediated but not E-selectin-mediated neutrophil rolling and migration.

Authors:  J Yang; T Hirata; K Croce; G Merrill-Skoloff; B Tchernychev; E Williams; R Flaumenhaft; B C Furie; B Furie
Journal:  J Exp Med       Date:  1999-12-20       Impact factor: 14.307

10.  Absence of trauma-induced leukocyte rolling in mice deficient in both P-selectin and intercellular adhesion molecule 1.

Authors:  E J Kunkel; U Jung; D C Bullard; K E Norman; B A Wolitzky; D Vestweber; A L Beaudet; K Ley
Journal:  J Exp Med       Date:  1996-01-01       Impact factor: 14.307
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