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Literature DB >> 768983

Stringent factor from Escherichia coli directs ribosomal binding and release of uncharged tRNA.

Abstract

Uncharged tRNA is preferentially bound to the peptidyl site of the ribosome in the absence of stringent factor ,but in its presence is directed to the acceptor site. The synthesis of pppGpp and ppGpp is initiated by tRNA bound in the acceptor but not in the peptidyl site. In this reaction, tRNA is not permanently attached to the acceptor site. Uncharged [32P] tRNA but not 3H-labeled stringent factor is released from the ribosome after each round of stringent factor-dependent hydrolysis of ATP. ATP-32PPi-exchange experiments reveal that exchange is independent of the presence of GTP but strongly enhanced by the addition of stringent factor and tRNA. The tRNA release is suppressed when ATP is replaced by beta, gamma-imido adenosine 5'-triphosphate, 5'-AMP, or GTP.

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Year: 1976 PMID： 768983 PMCID： PMC335987 DOI： 10.1073/pnas.73.3.707

Source DB: PubMed Journal: Proc Natl Acad Sci U S A ISSN： 0027-8424 Impact factor: 11.205

15 in total

1. Codon specific, tRNA dependent in vitro synthesis of ppGpp and pppGpp.

Authors: F S Pedersen; E Lund; N O Kjeldgaard
Journal: Nat New Biol Date: 1973-05-02

2. A new transfer RNA fragment reaction: Tp psi pCpGp bound to a ribosome-messenger RNA complex induces the synthesis of guanosine tetra- and pentaphosphates.

Authors: D Richter; V A Erdmann; M Sprinzl
Journal: Proc Natl Acad Sci U S A Date: 1974-08 Impact factor: 11.205

3. Synthesis of guanosine tetra- and pentaphosphate requires the presence of a codon-specific, uncharged transfer ribonucleic acid in the acceptor site of ribosomes.

Authors: W A Haseltine; R Block
Journal: Proc Natl Acad Sci U S A Date: 1973-05 Impact factor: 11.205

4. Inability of E. coli ribosomes to interact simultaneously with the bacterial elongation factors EF Tu and EF G.

Authors: D Richter
Journal: Biochem Biophys Res Commun Date: 1972-03-10 Impact factor: 3.575

5. Isolation and properties of a ribosome-bound factor required for ppGpp and ppGpp synthesis in Escherichia coli.

Authors: J W Cochran; R W Byrne
Journal: J Biol Chem Date: 1974-01-25 Impact factor: 5.157

6. The degradation of ribonucleic acids injected into Xenopus laevis oocytes.

Authors: C C Allende; J E Allende; R A Firtel
Journal: Cell Date: 1974-07 Impact factor: 41.582

7. The control of ribonucleic acid synthesis in Escherichia coli. V. Characterization of a nucleotide associated with the stringent response.

Authors: M Cashel; B Kalbacher
Journal: J Biol Chem Date: 1970-05-10 Impact factor: 5.157

8. The activation of amino acids for biosynthesis of gramicidin S.

Authors: W Gevers; H Kleinkauf; F Lipmann
Journal: Proc Natl Acad Sci U S A Date: 1968-05 Impact factor: 11.205

9. The interrelationship between guanosine triphosphatase and amino acid polymerization.

Authors: Y Nishizuka; F Lipmann
Journal: Arch Biochem Biophys Date: 1966-09-26 Impact factor: 4.013

10. Identification of the synthesis of guanosine tetraphosphate (MS I) as insertion of a pyrophosphoryl group into the 3'-position in guanosine 5'-diphosphate.

Authors: J Sy; F Lipmann
Journal: Proc Natl Acad Sci U S A Date: 1973-02 Impact factor: 11.205

13 in total

1. A cka-gfp transcriptional fusion reveals that the colicin K activity gene is induced in only 3 percent of the population.

Authors: Janez Mulec; Zdravko Podlesek; Peter Mrak; Andreja Kopitar; Alojz Ihan; Darja Zgur-Bertok
Journal: J Bacteriol Date: 2003-01 Impact factor: 3.490

2. The stimulation of Escherichia coli stringent factor-dependent synthesis of guanosine 3',5'-polyphosphate [(p)ppGpp] by rat liver ribosomal proteins.

Authors: S Fehr; A Lin; I G Wool; D Richter
Journal: Mol Gen Genet Date: 1979-11

3. Studies in vivo on Escherichia coli RNA polymerase mutants altered in the stringent response.

Authors: E Baracchini; R Glass; H Bremer
Journal: Mol Gen Genet Date: 1988-08

4. A cloned suppressor tRNA gene relaxes stringent control.

Authors: L Breeden; M Yarus; S Cline
Journal: Mol Gen Genet Date: 1980

5. New aspects of the ribosomal elongation cycle.

Authors: K H Nierhaus
Journal: Mol Cell Biochem Date: 1984 Impact factor: 3.396

6. Evidence that GCN1 and GCN20, translational regulators of GCN4, function on elongating ribosomes in activation of eIF2alpha kinase GCN2.

Authors: M J Marton; C R Vazquez de Aldana; H Qiu; K Chakraburtty; A G Hinnebusch
Journal: Mol Cell Biol Date: 1997-08 Impact factor: 4.272