Literature DB >> 7689718

A correlation between soluble brain nitric oxide synthase and NADPH-diaphorase activity is only seen after exposure of the tissue to fixative.

T Matsumoto1, M Nakane, J S Pollock, J E Kuk, U Förstermann.   

Abstract

In histochemical studies using fixed brain tissue, NADPH-diaphorase has been found to be colocalized with soluble nitric oxide synthase. In the present study, using fresh tissues from eight different regions of rat brain, NADPH-diaphorase activity was found mostly in the particulate fraction, whereas most of the nitric oxide synthase activity was located to the cytosolic fraction. Also, the distribution of NADPH-diaphorase activity among brain regions was different from that of nitric oxide synthase. Pretreatment of the fractions with paraformaldehyde virtually abolished the NADPH-diaphorase activity in the particulate fraction, whereas 40-60% of the NADPH-diaphorase activity remained intact in the cytosolic fraction. These results suggest that during fixation most NADPH-diaphorase activity is inactivated and only some of the NADPH-diaphorase activity associated with soluble nitric oxide synthase remains intact.

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Year:  1993        PMID： 7689718     DOI： 10.1016/0304-3940(93)90673-9

Source DB:  PubMed          Journal:  Neurosci Lett        ISSN： 0304-3940            Impact factor:   3.046


  41 in total

1.  NADPH-diaphorase activity and nitric oxide synthase isoforms in the trophoblast of Calomys callosus.

Authors:  N Moraes; D Zago; S Gagioti; M S Hoshida; E Bevilacqua
Journal:  J Anat       Date:  2001-04       Impact factor: 2.610

2.  Ultrastructural localisation of NADPH-d/nNOS expression in the superior cervical ganglion of the hamster.

Authors:  C Y Tseng; J H Lue; H M Chang; C Y Wen; J Y Shieh
Journal:  J Anat       Date:  2000-10       Impact factor: 2.610

3.  Regional distribution of binding sites for the nitric oxide synthase inhibitor L-[3H]nitroarginine in rat brain.

Authors:  V L Rao; R F Butterworth
Journal:  Neurochem Res       Date:  1996-03       Impact factor: 3.996

4.  Neuroplastic and neuropathological changes in the central nervous system of the Gray mussel Crenomytilus grayanus (Dunker) under environmental stress.

Authors:  Elena P Kotsyuba; Marina A Vaschenko
Journal:  Invert Neurosci       Date:  2010-05-26

5.  Nitric oxide synthase is not essential for Drosophila development.

Authors:  Nikita Yakubovich; Elizabeth A Silva; Patrick H O'Farrell
Journal:  Curr Biol       Date:  2010-02-23       Impact factor: 10.834

6.  Neuronal and endothelial nitric oxide synthase immunoreactivity and NADPH-diaphorase staining in rat and human pancreas: influence of fixation.

Authors:  J Wörl; M Wiesand; B Mayer; K R Greskötter; W L Neuhuber
Journal:  Histochemistry       Date:  1994-11

7.  Differential localization of neuronal nitric oxide synthase immunoreactivity and NADPH-diaphorase activity in the cat spinal cord.

Authors:  M A Vizzard; S L Erdman; J R Roppolo; U Förstermann; W C de Groat
Journal:  Cell Tissue Res       Date:  1994-11       Impact factor: 5.249

8.  Distribution of NADPH-diaphorase and nitric oxide synthase-containing neurons in the intramural ganglia of guinea pig urinary bladder.

Authors:  Y Zhou; C K Tan; E A Ling
Journal:  J Anat       Date:  1997-01       Impact factor: 2.610

9.  L-NNA inhibits the histochemical NADPH-d reaction in rat spinal cord neurons.

Authors:  D Blottner; H G Baumgarten
Journal:  Histochem Cell Biol       Date:  1995-05       Impact factor: 4.304

10.  Induction of nitric oxide synthase and microglial responses precede selective cell death induced by chronic impairment of oxidative metabolism.

Authors:  N Y Calingasan; L C Park; L L Calo; R R Trifiletti; S E Gandy; G E Gibson
Journal:  Am J Pathol       Date:  1998-08       Impact factor: 4.307
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