| Literature DB >> 7686510 |
D K Pokholok1, S O Gudima, D S Yesipov, V N Dobrynin, V O Rechinsky, S N Kochetkov.
Abstract
To investigate the biochemical basis of the HIV-1 resistance to AZT we obtained the RT mutant containing four amino acid substitutions by an oligonucleotide-directed mutagenesis technique. Enzymatic properties of the wild type and mutant RTs were compared. 'AZT-resistant' mutations in RT were shown to be associated with the reduced capability of AZT-TP to block the DNA- but not RNA-directed DNA synthesis.Entities:
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Year: 1993 PMID： 7686510 DOI： 10.1016/0014-5793(93)81080-j
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124