Literature DB >> 7686488

Structural analysis of two oligosaccharide bisphosphates isolated from the lipopolysaccharide of a recombinant strain of Escherichia coli F515 (Re chemotype) expressing the genus-specific epitope of Chlamydia lipopolysaccharide.

O Holst1, W Broer, J E Thomas-Oates, U Mamat, H Brade.   

Abstract

The lipopolysaccharide of the recombinant strain Escherichia coli F515-207, expressing the genus-specific epitope of Chlamydia lipopolysaccharide, was sequentially de-O- and de-N-acylated by mild hydrazinolysis and treatment with 4 M KOH, respectively, yielding two oligosaccharide bisphosphates which were isolated by high-performance anion-exchange chromatography and gel-permeation chromatography. Their structures were determined by chemical analysis, NMR spectroscopy, and mass spectrometry as alpha-Kdo-(2-4)-alpha-Kdo-(2-6)-beta-D-GlcN-(1-6)-alpha-D-GlcN 1,4'-P2 (tetrasaccharide bisphosphate) and alpha-Kdo-(2-8)-alpha-Kdo-(2-4)-alpha-Kdo-(2-6)-beta-D-GlcN-(1-6)-alpha- D-GlcN 1,4'-P2 (pentasaccharide bisphosphate).

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Year:  1993        PMID： 7686488     DOI： 10.1111/j.1432-1033.1993.tb17971.x

Source DB:  PubMed          Journal:  Eur J Biochem        ISSN： 0014-2956


  11 in total

1.  Groove-type recognition of chlamydiaceae-specific lipopolysaccharide antigen by a family of antibodies possessing an unusual variable heavy chain N-linked glycan.

Authors:  Omid Haji-Ghassemi; Sven Müller-Loennies; Radka Saldova; Mohankumar Muniyappa; Lore Brade; Pauline M Rudd; David J Harvey; Paul Kosma; Helmut Brade; Stephen V Evans
Journal:  J Biol Chem       Date:  2014-03-28       Impact factor: 5.157

2.  Analysis of cross-reactive and specific anti-carbohydrate antibodies against lipopolysaccharide from Chlamydophila psittaci.

Authors:  Sandra Gerstenbruch; Cory L Brooks; Paul Kosma; Lore Brade; C Roger Mackenzie; Stephen V Evans; Helmut Brade; Sven Müller-Loennies
Journal:  Glycobiology       Date:  2009-12-18       Impact factor: 4.313

3.  Molecular dynamics simulations of six different fully hydrated monomeric conformers of Escherichia coli re-lipopolysaccharide in the presence and absence of Ca2+.

Authors:  S Obst; M Kastowsky; H Bradaczek
Journal:  Biophys J       Date:  1997-03       Impact factor: 4.033

4.  Lipopolysaccharide smooth-rough phase variation in bacteria of the genus Chlamydia.

Authors:  M Lukácová; M Baumann; L Brade; U Mamat; H Brade
Journal:  Infect Immun       Date:  1994-06       Impact factor: 3.441

5.  Lipooligosaccharide of Campylobacter jejuni: similarity with multiple types of mammalian glycans beyond gangliosides.

Authors:  R Scott Houliston; Evgeny Vinogradov; Monika Dzieciatkowska; Jianjun Li; Frank St Michael; Marie-France Karwaski; Denis Brochu; Harold C Jarrell; Craig T Parker; Nobuhiro Yuki; Robert E Mandrell; Michel Gilbert
Journal:  J Biol Chem       Date:  2011-01-21       Impact factor: 5.157

6.  Structure and immunogenicity of the rough-type lipopolysaccharide from the periodontal pathogen Tannerella forsythia.

Authors:  Gerald Posch; Oleh Andrukhov; Evgeny Vinogradov; Buko Lindner; Paul Messner; Otto Holst; Christina Schäffer
Journal:  Clin Vaccine Immunol       Date:  2013-04-24

7.  Functional characterization of Gne (UDP-N-acetylglucosamine-4-epimerase), Wzz (chain length determinant), and Wzy (O-antigen polymerase) of Yersinia enterocolitica serotype O:8.

Authors:  José Antonio Bengoechea; Elise Pinta; Tiina Salminen; Clemens Oertelt; Otto Holst; Joanna Radziejewska-Lebrecht; Zofia Piotrowska-Seget; Reija Venho; Mikael Skurnik
Journal:  J Bacteriol       Date:  2002-08       Impact factor: 3.490

8.  WaaA of the hyperthermophilic bacterium Aquifex aeolicus is a monofunctional 3-deoxy-D-manno-oct-2-ulosonic acid transferase involved in lipopolysaccharide biosynthesis.

Authors:  Uwe Mamat; Helgo Schmidt; Eva Munoz; Buko Lindner; Koichi Fukase; Anna Hanuszkiewicz; Jing Wu; Timothy C Meredith; Ronald W Woodard; Rolf Hilgenfeld; Jeroen R Mesters; Otto Holst
Journal:  J Biol Chem       Date:  2009-06-22       Impact factor: 5.157

9.  Characterization of a neutralizing monoclonal antibody directed at the lipopolysaccharide of Chlamydia pneumoniae.

Authors:  E M Peterson; L M de la Maza; L Brade; H Brade
Journal:  Infect Immun       Date:  1998-08       Impact factor: 3.441

10.  Structural Studies of Lipopolysaccharide-defective Mutants from Brucella melitensis Identify a Core Oligosaccharide Critical in Virulence.

Authors:  Carolina Fontana; Raquel Conde-Álvarez; Jonas Ståhle; Otto Holst; Maite Iriarte; Yun Zhao; Vilma Arce-Gorvel; Seán Hanniffy; Jean-Pierre Gorvel; Ignacio Moriyón; Göran Widmalm
Journal:  J Biol Chem       Date:  2016-02-11       Impact factor: 5.157
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