Literature DB >> 7684069

Nicotinic receptor-mediated release of noradrenaline in the human neuroblastoma SH-SY5Y.

P F Vaughan1, D F Kaye, H L Reeve, S G Ball, C Peers.   

Abstract

Dimethylphenylpiperazinium iodide (a nicotinic agonist) evokes noradrenaline release from human neuroblastoma SH-SY5Y cells that have been pretreated with 12-O-tetradecanoylphorbol 13-acetate for 8 min. This effect of dimethylphenylpiperazinium iodide was inhibited by 1 microM mecamylamine but not by 1 microM atropine, which suggests that SH-SY5Y cells express nicotinic receptors coupled to the release of noradrenaline. Dimethylphenylpiperazinium iodide-evoked release was enhanced by 5 microM Bay K 8644 (an L-type calcium agonist) and inhibited by 1 microM nifedipine. Dimethylphenylpiperazinium iodide depolarised SH-SY5Y cells and enhanced the level of intracellular calcium in cells loaded with fura 2. The effects of dimethylphenylpiperazinium iodide on noradrenaline release, depolarisation, and intracellular calcium levels were all inhibited by 1 microM desmethylimipramine. The results of this study show that nicotinic receptors in SH-SY5Y cells stimulate noradrenaline release by activation of L-type calcium channels.

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Year:  1993        PMID： 7684069     DOI： 10.1111/j.1471-4159.1993.tb03501.x

Source DB:  PubMed          Journal:  J Neurochem        ISSN： 0022-3042            Impact factor:   5.372


  7 in total

Review 1.  The regulation of neurotransmitter secretion by protein kinase C.

Authors:  P F Vaughan; J H Walker; C Peers
Journal:  Mol Neurobiol       Date:  1998-10       Impact factor: 5.590

2.  Differential effect of nicotinic agonists on the [3H]norepinephrine release from rat hippocampal slices.

Authors:  J P Kiss; K Windisch; K De Oliveira; E C Hennings; A Mike; B K Szász
Journal:  Neurochem Res       Date:  2001-09       Impact factor: 3.996

3.  Cellular responses to nicotinic receptor activation are decreased after prolonged exposure to galantamine in human neuroblastoma cells.

Authors:  Jacques Barik; Federico Dajas-Bailador; Susan Wonnacott
Journal:  Br J Pharmacol       Date:  2005-08       Impact factor: 8.739

4.  Differential effects of chronic drug treatment on alpha3* and alpha7 nicotinic receptor binding sites, in hippocampal neurones and SH-SY5Y cells.

Authors:  D L Ridley; A Rogers; S Wonnacott
Journal:  Br J Pharmacol       Date:  2001-08       Impact factor: 8.739

5.  A role for protein kinase C subtypes alpha and epsilon in phorbol-ester-enhanced K(+)- and carbachol-evoked noradrenaline release from the human neuroblastoma SH-SY5Y.

Authors:  N A Turner; M G Rumsby; J H Walker; F A McMorris; S G Ball; P F Vaughan
Journal:  Biochem J       Date:  1994-01-15       Impact factor: 3.857

6.  Enhancement of Ca2+ channel currents in human neuroblastoma (SH-SY5Y) cells by phorbol esters with and without activation of protein kinase C.

Authors:  H L Reeve; P F Vaughan; C Peers
Journal:  Pflugers Arch       Date:  1995-03       Impact factor: 3.657

7.  Muscarinic (M1) receptor-mediated inhibition of K(+)-evoked [3H]-noradrenaline release from human neuroblastoma (SH-SY5Y) cells via inhibition of L- and N-type Ca2+ channels.

Authors:  R L McDonald; P F Vaughan; C Peers
Journal:  Br J Pharmacol       Date:  1994-10       Impact factor: 8.739
  7 in total

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