Literature DB >> 7683195

A Staphylococcus aureus-specific oligonucleotide probe derived from 16S rRNA gene sequences.

R W Bentley1, N M Harland, J A Leigh, M D Collins.   

Abstract

Genes encoding 16S rRNA were sequenced from 16 species of Staphylococcus. Sequence analysis highlighted a potential Staph. aureus-specific region and a complementary oligonucleotide probe was synthesized and its specificity tested. Northern blotting indicated molecular specificity, and dot blots to RNA from Staph. aureus, Staph. capitis, Staph. caprae, Staph. carnosus, Staph. caseolyticus, Staph. cohnii, Staph. epidermidis, Staph. gallinarum, Staph. haemolyticus, Staph. hominis, Staph. hyicus, Staph. saprophyticus, Staph. sciuri, Staph. simulans, Staph. warneri and Staph. xylosus indicated species-specificity.

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Year:  1993        PMID: 7683195     DOI: 10.1111/j.1472-765x.1993.tb01397.x

Source DB:  PubMed          Journal:  Lett Appl Microbiol        ISSN: 0266-8254            Impact factor:   2.858


  4 in total

1.  Rapid identification of bacteria in blood cultures by using fluorescently labeled oligonucleotide probes.

Authors:  G J Jansen; M Mooibroek; J Idema; H J Harmsen; G W Welling; J E Degener
Journal:  J Clin Microbiol       Date:  2000-02       Impact factor: 5.948

2.  Detection of Staphylococcus aureus and Staphylococcus epidermidis in clinical samples by 16S rRNA-directed in situ hybridization.

Authors:  V Krimmer; H Merkert; C von Eiff; M Frosch; J Eulert; J F Löhr; J Hacker; W Ziebuhr
Journal:  J Clin Microbiol       Date:  1999-08       Impact factor: 5.948

Review 3.  Molecular diagnostics of clinically important staphylococci.

Authors:  J Stepán; R Pantůcek; J Doskar
Journal:  Folia Microbiol (Praha)       Date:  2004       Impact factor: 2.099

4.  Rapid identification of Staphylococcus aureus directly from blood cultures by fluorescence in situ hybridization with peptide nucleic acid probes.

Authors:  Kenneth Oliveira; Gary W Procop; Deborah Wilson; James Coull; Henrik Stender
Journal:  J Clin Microbiol       Date:  2002-01       Impact factor: 5.948

  4 in total

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