Dose-dependency of radiation on enzyme production in Trichoderma reesei.

Effect of irradiation dose on the production of cellulase and amylase related enzymes in Trichoderma reesei was studied, in which post-irradiation time response pattern was measured. The damage of the cells irradiated with certain irradiation doses (1.40 +/- 0.20 x 10(5), 2.20 +/- 0.10 x 10(5), 3.00 +/- 0.50 x 10(5) and 3.50 +/- 0.20 x 10(5) rad) was rapidly recovered. The increased enzyme production in the culture of the irradiated cells resulted from the recovery of radiation damage after irradiation. The function of cell growth was not affected by irradiation below dose of 5 x 10(5) rad, though the function of enzyme synthesis was drastically affected.