| Literature DB >> 7679184 |
M B Villiers1, F M Gabert, M R Jacquier, C L Villiers, M G Colomb.
Abstract
Tetanus toxin contains a metal-binding site for zinc, located in its light chain. The sequence accounting for Zn fixation is part of a predicted amphipathic helical secondary structure and corresponds to a putative T cell epitope according to Rothbard and Taylor (EMBO J. 7, 93-100, 1988). In this paper, we analyse the antigenic properties of two synthetic peptides (233-248 = P12 and 225-243 = P13) containing the Zn binding sequence. Our results show that peptide P13 contains a B and T epitope. The B epitope seems to be immuno-dominant whether the T epitope is at least DR2 restricted. Zn binding on P13 leads to a decrease in its recognition by both antibodies and T lymphocytes.Entities:
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Year: 1993 PMID： 7679184 DOI： 10.1016/0161-5890(93)90084-o
Source DB: PubMed Journal: Mol Immunol ISSN： 0161-5890 Impact factor: 4.407