| Literature DB >> 7679080 |
R Berendes1, A Burger, D Voges, P Demange, R Huber.
Abstract
A new assay for the calcium channel activity of annexin V was developed. The calcium-sensitive fluorescence indicator, fura-2, was incorporated into large unilamellar vesicles (LUV). After establishing a calcium gradient across the liposomal membranes, native or mutated annexin V was added. The resulting calcium influx into the LUV detected through the fluorescence changes of fura-2 was used as a qualitative test for the electrophysiological properties of annexin V.Entities:
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Year: 1993 PMID： 7679080 DOI： 10.1016/0014-5793(93)81507-v
Source DB: PubMed Journal: FEBS Lett ISSN： 0014-5793 Impact factor: 4.124