| Literature DB >> 7678995 |
K E Langley1, L G Bennett, J Wypych, S A Yancik, X D Liu, K R Westcott, D G Chang, K A Smith, K M Zsebo.
Abstract
Stem cell factor (SCF) is a recently described factor active in the early stages of hematopoiesis. It can exist in membrane-bound form and in proteolytically released soluble form. The levels and nature of SCF in human serum are described. As determined by an enzyme-linked immunosorbent assay performed for 257 samples, SCF level in serum averaged 3.3 +/- 1.1 ng/mL. The serum SCF was partially purified by immunoaffinity chromatography and analyzed by glycosidase treatments in conjunction with sodium dodecyl sulfate-polyacrylamide gel electrophoresis and immunoblotting. The results show that the SCF has N-linked and O-linked carbohydrate and corresponds to the soluble form, at or about 165 amino acids in length. The findings suggest functional importance for soluble SCF in humans.Entities:
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Year: 1993 PMID： 7678995
Source DB: PubMed Journal: Blood ISSN： 0006-4971 Impact factor: 22.113