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Literature DB >> 7678733

Ca2+/calmodulin-dependent nitric oxide synthase activity in the human cervix carcinoma cell line ME-180.

G Werner-Felmayer¹, E R Werner, D Fuchs, A Hausen, B Mayer, G Reibnegger, G Weiss, H Wachter.

Abstract

We show here that the human cervix carcinoma cell line ME-180 expresses a constitutive nitric oxide (NO) synthase, as demonstrated by formation of [3H]citrulline and nitrite. The enzyme is dependent on tetrahydrobiopterin, NADPH, flavins and Ca2+/calmodulin. Enzyme activity is located in the cytosol rather than in the membrane fraction and can be inhibited by NG-monomethyl-L-arginine (NMMA). An antiserum to NO synthase purified from porcine cerebellum inhibited the enzyme activity. ME-180 cells released NO, as was shown by stimulation of guanylate cyclase (EC 4.6.1.2) in RFL-6 detector cells; this release was stimulated 8-fold by the Ca2+ ionophore A23187 and 2-fold by increasing the intracellular tetrahydrobiopterin levels with cytokines. This is the first characterization of a Ca2+/calmodulin-dependent NO synthase activity in human epithelial-type tumour cells.

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Year: 1993 PMID： 7678733 PMCID： PMC1132175 DOI： 10.1042/bj2890357

Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857

36 in total

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Authors: D J Stuehr; H J Cho; N S Kwon; M F Weise; C F Nathan
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5. 7-Substituted pterins: formation during phenylalanine hydroxylation in the absence of dehydratase.

Authors: H C Curtius; C Adler; I Rebrin; C Heizmann; S Ghisla
Journal: Biochem Biophys Res Commun Date: 1990-11-15 Impact factor: 3.575

6. An L-arginine/nitric oxide pathway present in human platelets regulates aggregation.

Authors: M W Radomski; R M Palmer; S Moncada
Journal: Proc Natl Acad Sci U S A Date: 1990-07 Impact factor: 11.205

7. Human colorectal adenocarcinoma cells: differential nitric oxide synthesis determines their ability to aggregate platelets.

Authors: M W Radomski; D C Jenkins; L Holmes; S Moncada
Journal: Cancer Res Date: 1991-11-15 Impact factor: 12.701

8. Brain nitric oxide synthase is a biopterin- and flavin-containing multi-functional oxido-reductase.

Authors: B Mayer; M John; B Heinzel; E R Werner; H Wachter; G Schultz; E Böhme
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9. Calmodulin-dependent endothelium-derived relaxing factor/nitric oxide synthase activity is present in the particulate and cytosolic fractions of bovine aortic endothelial cells.

Authors: U Förstermann; J S Pollock; H H Schmidt; M Heller; F Murad
Journal: Proc Natl Acad Sci U S A Date: 1991-03-01 Impact factor: 11.205

10. Calmodulin-independent nitric oxide synthase from rat polymorphonuclear neutrophils.

Authors: Y Yui; R Hattori; K Kosuga; H Eizawa; K Hiki; S Ohkawa; K Ohnishi; S Terao; C Kawai
Journal: J Biol Chem Date: 1991-02-25 Impact factor: 5.157

10 in total

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2. Tetrahydrobiopterin alters superoxide and nitric oxide release in prehypertensive rats.

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Journal: J Clin Invest Date: 1998-04-01 Impact factor: 14.808

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Journal: Naunyn Schmiedebergs Arch Pharmacol Date: 1995-05 Impact factor: 3.000

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Authors: Clinton R Nishida; Paul R Ortiz de Montellano
Journal: J Med Chem Date: 2008-08-06 Impact factor: 7.446

5. Nitric oxide signaling to iron-regulatory protein: direct control of ferritin mRNA translation and transferrin receptor mRNA stability in transfected fibroblasts.

Authors: K Pantopoulos; M W Hentze
Journal: Proc Natl Acad Sci U S A Date: 1995-02-28 Impact factor: 11.205