BACKGROUND: The MUC2 intestinal mucin gene contains tandem repeats of 23 amino acid length that are rich in threonine. METHODS: Mouse monoclonal antibody LDQ10 was raised against chemically deglycosylated mucin isolated from LS174T colon cancer nude mouse xenografts. RESULTS: LDQ10 reacts with deglycosylated colon cancer mucin and with a synthetic peptide encompassing the MUC2 tandem repeat sequence. In immunohistochemical assays, strong reactivity with goblet cells in colon, small bowel, and stomach is observed; weaker reactivity with mucin-producing cells in other epithelial tissues is shown. The epitope recognized by LDQ10 is localized in the rough endoplasmic reticulum of normal colonic goblet cells. LDQ10 also shows strong reactivity with colorectal and stomach cancers and weaker reactivity with pancreas, breast, and bladder cancers. CONCLUSIONS: Antibody LDQ10 detects a peptide epitope of MUC2 that becomes cryptic on glycosylation. Altered synthesis of the MUC2 apomucin takes place in a variety of epithelial cancers.
BACKGROUND: The MUC2 intestinal mucin gene contains tandem repeats of 23 amino acid length that are rich in threonine. METHODS:Mouse monoclonal antibody LDQ10 was raised against chemically deglycosylated mucin isolated from LS174T colon cancer nude mouse xenografts. RESULTS: LDQ10 reacts with deglycosylated colon cancer mucin and with a synthetic peptide encompassing the MUC2 tandem repeat sequence. In immunohistochemical assays, strong reactivity with goblet cells in colon, small bowel, and stomach is observed; weaker reactivity with mucin-producing cells in other epithelial tissues is shown. The epitope recognized by LDQ10 is localized in the rough endoplasmic reticulum of normal colonic goblet cells. LDQ10 also shows strong reactivity with colorectal and stomach cancers and weaker reactivity with pancreas, breast, and bladder cancers. CONCLUSIONS: Antibody LDQ10 detects a peptide epitope of MUC2 that becomes cryptic on glycosylation. Altered synthesis of the MUC2 apomucin takes place in a variety of epithelial cancers.
Authors: A López-Ferrer; C de Bolós; C Barranco; M Garrido; J Isern; I Carlstedt; C A Reis; J Torrado; F X Real Journal: Gut Date: 2000-09 Impact factor: 23.059
Authors: C A Reis; T Sørensen; U Mandel; L David; E Mirgorodskaya; P Roepstorff; J Kihlberg; J E Hansen; H Clausen Journal: Glycoconj J Date: 1998-01 Impact factor: 2.916
Authors: G Latella; R Fonti; R Caprilli; A Marcheggiano; F Magliocca; K M Das; G Gambús; Y Sambuy Journal: Int J Colorectal Dis Date: 1996 Impact factor: 2.571
Authors: Erika López-Arribillaga; Verónica Rodilla; Carlota Colomer; Anna Vert; Amy Shelton; Jason H Cheng; Bing Yan; Abel Gonzalez-Perez; Melissa R Junttila; Mar Iglesias; Ferran Torres; Joan Albanell; Alberto Villanueva; Anna Bigas; Christian W Siebel; LLuís Espinosa Journal: Nat Commun Date: 2018-07-31 Impact factor: 14.919