Literature DB >> 7675038

Rapid isolation of any known genes from whole cells of yeast by PCR.

D Ramotar1.   

Abstract

A short protocol was developed that allows the rapid isolation of any known Saccharomyces cerevisiae gene. Two known genes, APN1 and IMP2, were isolated directly from whole cells of yeast using polymerase chain reaction, without the need for purified template genomic DNA.

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Year:  1995        PMID： 7675038     DOI： 10.1007/bf00935491

Source DB:  PubMed          Journal:  Mol Cell Biochem        ISSN： 0300-8177            Impact factor:   3.396


  3 in total

1.  Targeting, disruption, replacement, and allele rescue: integrative DNA transformation in yeast.

Authors:  R Rothstein
Journal:  Methods Enzymol       Date:  1991       Impact factor: 1.600

2.  IMP2, a nuclear gene controlling the mitochondrial dependence of galactose, maltose and raffinose utilization in Saccharomyces cerevisiae.

Authors:  C Donnini; T Lodi; I Ferrero; P P Puglisi
Journal:  Yeast       Date:  1992-02       Impact factor: 3.239

3.  Intracellular localization of the Apn1 DNA repair enzyme of Saccharomyces cerevisiae. Nuclear transport signals and biological role.

Authors:  D Ramotar; C Kim; R Lillis; B Demple
Journal:  J Biol Chem       Date:  1993-09-25       Impact factor: 5.157
  3 in total
  2 in total

1.  Direct PCR on fruitflies and blood flukes without prior DNA isolation.

Authors:  C G Grevelding; A Kampkötter; M Hollmann; U Schäfer; W Kunz
Journal:  Nucleic Acids Res       Date:  1996-10-15       Impact factor: 16.971

2.  The Saccharomyces cerevisiae IMP2 gene encodes a transcriptional activator that mediates protection against DNA damage caused by bleomycin and other oxidants.

Authors:  J Y Masson; D Ramotar
Journal:  Mol Cell Biol       Date:  1996-05       Impact factor: 4.272
  2 in total

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