OBJECTIVE: To determine whether women with Chlamydia trachomatis-associated tubal infertility are more likely than other infertile women to have antibodies to a particular region of the 60-kd chlamydial heat shock protein, hsp60. DESIGN: Serologic responses to the chlamydial hsp60 were examined in 43 infertile women seropositive for Chlamydia trachomatis, including 21 women with tubal infertility, 13 women with endometriosis, and 9 women with other causes of infertility. Antibody responses were localized to regions of hsp60 using five nonoverlapping recombinant polypeptides. RESULTS: Sixteen women with tubal infertility had anti-hsp60 antibodies compared with seven women with endometriosis and two women with other causes of infertility. Antibodies of 11 women with tubal infertility reacted predominantly with a region of hsp60 containing amino acids (201 to 300) compared with 1 women without tubal infertility. In contrast, antibodies that localized to the carboxyl terminus, amino acids (401 to 544), were seen equally in all groups. CONCLUSIONS: Among seropositive infertile women, antibodies that localized to amino acids (201 to 300) were immunodominant in those with tubal infertility but not in those with infertility due to other causes.
OBJECTIVE: To determine whether women with Chlamydia trachomatis-associated tubal infertility are more likely than other infertile women to have antibodies to a particular region of the 60-kd chlamydial heat shock protein, hsp60. DESIGN: Serologic responses to the chlamydial hsp60 were examined in 43 infertile women seropositive for Chlamydia trachomatis, including 21 women with tubal infertility, 13 women with endometriosis, and 9 women with other causes of infertility. Antibody responses were localized to regions of hsp60 using five nonoverlapping recombinant polypeptides. RESULTS: Sixteen women with tubal infertility had anti-hsp60 antibodies compared with seven women with endometriosis and two women with other causes of infertility. Antibodies of 11 women with tubal infertility reacted predominantly with a region of hsp60 containing amino acids (201 to 300) compared with 1 women without tubal infertility. In contrast, antibodies that localized to the carboxyl terminus, amino acids (401 to 544), were seen equally in all groups. CONCLUSIONS: Among seropositive infertile women, antibodies that localized to amino acids (201 to 300) were immunodominant in those with tubal infertility but not in those with infertility due to other causes.
Authors: Allison K Rodgers; Jie Wang; Yingqian Zhang; Alan Holden; Blake Berryhill; Nicole M Budrys; Robert S Schenken; Guangming Zhong Journal: Am J Obstet Gynecol Date: 2010-11 Impact factor: 8.661
Authors: Allison K Rodgers; Nicole M Budrys; Siqi Gong; Jie Wang; Alan Holden; Robert S Schenken; Guangming Zhong Journal: Fertil Steril Date: 2011-07-13 Impact factor: 7.329
Authors: Chunmei Cheng; Pooja Jain; Sukumar Pal; Delia Tifrea; Guifeng Sun; Andy A Teng; Xiaowu Liang; Philip L Felgner; Luis M de la Maza Journal: Microbes Infect Date: 2013-10-23 Impact factor: 2.700
Authors: Michele D Picard; Jean-Luc Bodmer; Todd M Gierahn; Alexander Lee; Jessica Price; Kenya Cohane; Veronica Clemens; Victoria L DeVault; Galina Gurok; Robert Kohberger; Darren E Higgins; George R Siber; Jessica Baker Flechtner; William M Geisler Journal: Clin Vaccine Immunol Date: 2015-10-07