| Literature DB >> 7670520 |
C Labriola1, M Sousa, J J Cazzulo.
Abstract
The major cysteine proteinase from Trypanosoma cruzi, cruzipain, can be obtained essentially homogeneous, starting from crude epimastigote extracts, in one step, by affinity chromatography on Cystatin-Sepharose (specific for cysteine proteinases) or ConA-Sepharose (specific for high mannose N-linked glycoproteins). The methods offer considerable potential for enzyme purification from scarce sources, such as other parasite stages or radioactively labelled material with high specific radioactivity.Entities:
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Year: 1993 PMID： 7670520
Source DB: PubMed Journal: Biol Res ISSN： 0716-9760 Impact factor: 5.612