Literature DB >> 7670198

Nucleotide sequence of alpha-galactosidase cDNA from Mortierella vinacea.

H Shibuya1, H Kobayashi, K Kasamo, I Kusakabe.   

Abstract

To analyze the primary structure of Mortierella vinacea alpha-galactosidase, a cDNA library of M. vinacea mRNA in lambda gt10 was constructed. A clone, which has an insert size of about 1.4 kilobase pairs, was found to contain the coding region of the mature enzyme. The deduced amino acid sequence share that the mature enzyme consisted of 397 amino acid residues with a molecular mass of 44,350 Da. The sequence identity of the mature enzyme with alpha-galactosidases from Saccharomyces carlsbergensis, Cyamopsis tetragonoloba (guar), and human were 47%, 43%, and 34%, respectively.

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Year:  1995        PMID： 7670198     DOI： 10.1271/bbb.59.1345

Source DB:  PubMed          Journal:  Biosci Biotechnol Biochem        ISSN： 0916-8451            Impact factor:   2.043


  4 in total

1.  Purification and characterization of the recombinant Thermus sp. strain T2 alpha-galactosidase expressed in Escherichia coli.

Authors:  M Ishiguro; S Kaneko; A Kuno; Y Koyama; S Yoshida; G G Park; Y Sakakibara; I Kusakabe; H Kobayashi
Journal:  Appl Environ Microbiol       Date:  2001-04       Impact factor: 4.792

2.  alpha-Galactosidase Aga27A, an enzymatic component of the Clostridium josui cellulosome.

Authors:  Sadanari Jindou; Shuichi Karita; Emi Fujino; Tsuchiyoshi Fujino; Hidenori Hayashi; Tetsuya Kimura; Kazuo Sakka; Kunio Ohmiya
Journal:  J Bacteriol       Date:  2002-01       Impact factor: 3.490

3.  Differential expression of three alpha-galactosidase genes and a single beta-galactosidase gene from Aspergillus niger.

Authors:  R P de Vries; H C van den Broeck; E Dekkers; P Manzanares; L H de Graaff; J Visser
Journal:  Appl Environ Microbiol       Date:  1999-06       Impact factor: 4.792

4.  Cloning and high-level expression of alpha-galactosidase cDNA from Penicillium purpurogenum.

Authors:  H Shibuya; H Nagasaki; S Kaneko; S Yoshida; G G Park; I Kusakabe; H Kobayashi
Journal:  Appl Environ Microbiol       Date:  1998-11       Impact factor: 4.792
  4 in total

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