Literature DB >> 7667882

Purification of His-Tag fusion proteins from Escherichia coli.

P Hengen1.   

Abstract

Methods and reagents is a unique monthly column that highlights current discussions in the newsgroup bionet.molbio.methds-reagnts, available on the Internet. This month's column discusses a contaminating protein from Escherichia coli found when histidine-tagged fusion proteins are purified by metal chelate affinity chromatography. For details on how to partake in the newsgroup, see the accompanying box.

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Year:  1995        PMID： 7667882     DOI： 10.1016/s0968-0004(00)89045-3

Source DB:  PubMed          Journal:  Trends Biochem Sci        ISSN： 0968-0004            Impact factor:   13.807


  27 in total

1.  Deoxy- and dideoxynucleotide discrimination and identification of critical 5' nuclease domain residues of the DNA polymerase I from Mycobacterium tuberculosis.

Authors:  V Mizrahi; P Huberts
Journal:  Nucleic Acids Res       Date:  1996-12-15       Impact factor: 16.971

2.  Physiological characterization of ATP-citrate lyase in Aspergillus niger.

Authors:  Hong Chen; Xihong He; Hongran Geng; Hao Liu
Journal:  J Ind Microbiol Biotechnol       Date:  2014-02-25       Impact factor: 3.346

3.  Enhanced purification of histidine-tagged carboxymethylcellulase produced by Escherichia coli BL21/LBH-10 and comparison of its characteristics with carboxymethylcellulase without histidine-tag.

Authors:  Duk-Un Kang; Yong-Suk Lee; Jin-Woo Lee
Journal:  Mol Biol Rep       Date:  2019-02-02       Impact factor: 2.316

4.  Genetic and functional characterization of the hyaluronate lyase HylB and the beta-N-acetylglucosaminidase HylZ in Streptococcus zooepidemicus.

Authors:  Xiaqing Sun; Zhen Wang; Yali Bi; Yangyang Wang; Hao Liu
Journal:  Curr Microbiol       Date:  2014-08-22       Impact factor: 2.188

5.  Identification of the coding region for a second poly(A) polymerase in Escherichia coli.

Authors:  G J Cao; J Pogliano; N Sarkar
Journal:  Proc Natl Acad Sci U S A       Date:  1996-10-15       Impact factor: 11.205

6.  Mutagenesis of the active site of the human Theta-class glutathione transferase GSTT2-2: catalysis with different substrates involves different residues.

Authors:  K L Tan; G Chelvanayagam; M W Parker; P G Board
Journal:  Biochem J       Date:  1996-10-01       Impact factor: 3.857

Review 7.  Molecular Probes, Chemosensors, and Nanosensors for Optical Detection of Biorelevant Molecules and Ions in Aqueous Media and Biofluids.

Authors:  Joana Krämer; Rui Kang; Laura M Grimm; Luisa De Cola; Pierre Picchetti; Frank Biedermann
Journal:  Chem Rev       Date:  2022-01-07       Impact factor: 60.622

8.  Building blocks for protein interaction devices.

Authors:  Raik Grünberg; Tony S Ferrar; Almer M van der Sloot; Marco Constante; Luis Serrano
Journal:  Nucleic Acids Res       Date:  2010-03-09       Impact factor: 16.971

9.  Characterization and in vivo functional analysis of the Schizosaccharomyces pombe ICLN gene.

Authors:  Adrien Barbarossa; Etienne Antoine; Henry Neel; Thierry Gostan; Johann Soret; Rémy Bordonné
Journal:  Mol Cell Biol       Date:  2013-12-02       Impact factor: 4.272

10.  Novel role of ZmaNAC36 in co-expression of starch synthetic genes in maize endosperm.

Authors:  Junjie Zhang; Jiang Chen; Qiang Yi; Yufeng Hu; Hanmei Liu; Yinghong Liu; Yubi Huang
Journal:  Plant Mol Biol       Date:  2014-02       Impact factor: 4.076
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