Literature DB >> 766750

Evidence for an active dimer of Escherichia coli beta-galactosidase.

C M Kaneshiro, C A Enns, M G Hahn, J S Peterson, F J Reithel.   

Abstract

BETA-Galactosidase (EC 3.2.1.23), prepared from strains ML 308 and K12 3300 of Escherichia coli, dissociated into an inactive monomer in the presence of Ag+. When such a monomer preparation is treated with excess of thiol an enzymically active dimer is formed in addition to an active tetramer. It is suggested that Ag+ may be of value in studies on other multimeric proteins as a mild dissociating agent.

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Year:  1975        PMID： 766750      PMCID： PMC1172374          DOI： 10.1042/bj1510433

Source DB:  PubMed          Journal:  Biochem J        ISSN： 0264-6021            Impact factor:   3.857


  5 in total

1.  Studies on protein multimers. V. Further characterization of beta-galactosidase self-association behavior with observations on apparent specific volume.

Authors:  C C Contaxis; D McAfee; R Goodrich; F J Reithel
Journal:  Int J Pept Protein Res       Date:  1973

2.  A study of the sulfhydryl groups of the catalytic subunit of Escherichia coli aspartate transcarbamylase. The use of enzyme--5-thio-2-nitrobenzoate mixed disulfides as intermediates in modifying enzyme sulfhydryl groups.

Authors:  T C Vanaman; G R Stark
Journal:  J Biol Chem       Date:  1970-07-25       Impact factor: 5.157

3.  Purification and characterization of the multiple forms of beta-galactosidase of Escherichia coli.

Authors:  S L Marchesi; E Steers; S Shifrin
Journal:  Biochim Biophys Acta       Date:  1969-05

4.  Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads.

Authors:  P Cuatrecasas
Journal:  J Biol Chem       Date:  1970-06       Impact factor: 5.157

5.  Studies on protein multimers. The association-dissociation behaviour of -galactosidase in glycerol.

Authors:  C C Contaxis; F J Reithel
Journal:  Biochem J       Date:  1971-09       Impact factor: 3.857
  5 in total
  3 in total

1.  A system for quantifying dynamic protein interactions defines a role for Herceptin in modulating ErbB2 interactions.

Authors:  T S Wehrman; W J Raab; C L Casipit; R Doyonnas; J H Pomerantz; H M Blau
Journal:  Proc Natl Acad Sci U S A       Date:  2006-12-05       Impact factor: 11.205

2.  Direct visualization of ligand-protein interactions using atomic force microscopy.

Authors:  Calum S Neish; Ian L Martin; Robert M Henderson; J Michael Edwardson
Journal:  Br J Pharmacol       Date:  2002-04       Impact factor: 8.739

3.  Immobilization of enzymes to silver island films for enhanced enzymatic activity.

Authors:  Biebele Abel; Kadir Aslan
Journal:  J Colloid Interface Sci       Date:  2013-10-24       Impact factor: 8.128
  3 in total

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