Literature DB >> 7667074

Expression of a renal Na/Pi cotransporter (NaPi-1) in MDCK and LLC-PK1 cells.

E S Quabius1, H Murer, J Biber.   

Abstract

We have recently isolated a corresponding DNA (cDNA) (NaPi-1) which, most likely is related to rabbit renal proximal tubular Na/Pi cotransport. In the present study we have stably transfected Madin Darby canine kidney (MDCK) and LLC-PK1 cells with a vector containing the NaPi-1 cDNA under the control of a dexamethasone-inducible promoter. In transfected MDCK and LLC-PK1 cells, NaPi-1-related mRNA could be detected by reverse transcription-polymerase chain reaction (RT-PCR) only after induction of the cells by dexamethasone. Similarly an increase in Na(+)-dependent Pi uptake was observed in NaPi-1 transfected MDCK and LLC-PK1 cells grown in Petri dishes and stimulated by dexamethasone; in cells grown on filter supports, dexamethasone-induced transport activity in NaPi-1 transfectants was only observed at the apical cell surface.

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Year:  1995        PMID： 7667074     DOI： 10.1007/bf00373848

Source DB:  PubMed          Journal:  Pflugers Arch        ISSN： 0031-6768            Impact factor:   3.657


  20 in total

1.  A simple technique for eliminating interference by detergents in the Lowry method of protein determination.

Authors:  J R Dulley; P A Grieve
Journal:  Anal Biochem       Date:  1975-03       Impact factor: 3.365

2.  Characterization of basolateral Na/H exchange (Na/H-1) in MDCK cells.

Authors:  S Vilella; L Guerra; C Helmle-Kolb; H Murer
Journal:  Pflugers Arch       Date:  1992-03       Impact factor: 3.657

3.  Neutral endopeptidase, a major brush border protein of the kidney proximal nephron, is directly targeted to the apical domain when expressed in Madin-Darby canine kidney cells.

Authors:  F Jalal; G Lemay; M Zollinger; A Berteloot; G Boileau; P Crine
Journal:  J Biol Chem       Date:  1991-10-15       Impact factor: 5.157

4.  cDNA cloning and expression in Xenopus laevis oocytes of pig renal dipeptidase, a glycosyl-phosphatidylinositol-anchored ectoenzyme.

Authors:  E Rached; N M Hooper; P James; G Semenza; A J Turner; N Mantei
Journal:  Biochem J       Date:  1990-11-01       Impact factor: 3.857

Review 5.  Epithelial cells in tissue culture.

Authors:  G J Gstraunthaler
Journal:  Ren Physiol Biochem       Date:  1988 Jan-Feb

6.  Targeting of recombinant Na+/glucose cotransporter (SGLT1) to the apical membrane.

Authors:  C T Kong; A Varde; J E Lever
Journal:  FEBS Lett       Date:  1993-10-25       Impact factor: 4.124

7.  Role of microtubules in the adaptive response to low phosphate of Na/Pi cotransport in opossum kidney cells.

Authors:  E Hansch; J Forgo; H Murer; J Biber
Journal:  Pflugers Arch       Date:  1993-02       Impact factor: 3.657

8.  Localization of NaPi-1, a Na/Pi cotransporter, in rabbit kidney proximal tubules. II. Localization by immunohistochemistry.

Authors:  J Biber; M Custer; A Werner; B Kaissling; H Murer
Journal:  Pflugers Arch       Date:  1993-08       Impact factor: 3.657

9.  Localization of NaPi-1, a Na-Pi cotransporter, in rabbit kidney proximal tubules. I. mRNA localization by reverse transcription/polymerase chain reaction.

Authors:  M Custer; F Meier; E Schlatter; R Greger; A Garcia-Perez; J Biber; H Murer
Journal:  Pflugers Arch       Date:  1993-08       Impact factor: 3.657

Review 10.  Renal sodium-phosphate cotransport.

Authors:  H Murer; J Biber
Journal:  Curr Opin Nephrol Hypertens       Date:  1994-09       Impact factor: 2.894
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