| Literature DB >> 7664093 |
M Klemba1, K H Gardner, S Marino, N D Clarke, L Regan.
Abstract
We describe the successful design of a tetrahedral His3Cys Zn(II)-binding site in a small protein of known structure: the B1 domain of Streptococcal protein G. The B1 variants containing the novel metal-binding site were characterized using a combination of optical absorption, circular dichroism and NMR spectroscopies. The results indicate that the designed proteins bind Zn(II) with high affinity and tetrahedral coordination geometry, and that the overall secondary and tertiary structure of the B1 domain is maintained.Entities:
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Year: 1995 PMID： 7664093 DOI： 10.1038/nsb0595-368
Source DB: PubMed Journal: Nat Struct Biol ISSN： 1072-8368