Literature DB >> 7664060

Formation and properties of a stable 'high-potential' copper-iron-sulphur cluster in a ferredoxin.

J N Butt1, J Niles, F A Armstrong, J Breton, A J Thomson.   

Abstract

A ferredoxin isolated from Desulfovibrio africanus contains a [3Fe-4S] cluster that reversibly binds a copper atom, yielding a stable product with a greatly increased reduction potential. The reaction is readily detected in protein molecules adsorbed as a film on an electrode surface. Electron paramagnetic resonance (EPR) and magnetic circular dichroism (MCD) spectra of oxidized and reduced bulk solution products support their assignment as [Cu3Fe-4S]2+ (S = 1/2) and [Cu3Fe-4S]1+ (S = 2) respectively, with copper bound formally as Cu(I). Cyanide causes selective loss of copper and regeneration of the [3Fe-4S] reactant. The results demonstrate the chemical feasibility of CuFeS clusters and suggest that they could exist naturally in biological systems.

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Year:  1994        PMID： 7664060     DOI： 10.1038/nsb0794-427

Source DB:  PubMed          Journal:  Nat Struct Biol        ISSN： 1072-8368


  6 in total

1.  [3Fe-4S] <--> [4Fe-4S] cluster interconversion in Desulfovibrio africanus ferredoxin III: properties of an Asp14 --> Cys mutant.

Authors:  J L Busch; J L Breton; B M Bartlett; F A Armstrong; R James; A J Thomson
Journal:  Biochem J       Date:  1997-04-01       Impact factor: 3.857

2.  Heterometallic [AgFe(3)S (4)] ferredoxin variants: synthesis, characterization, and the first crystal structure of an engineered heterometallic iron-sulfur protein.

Authors:  Maja Martic; Ida Noémi Jakab-Simon; Lærke Tvedebrink Haahr; Wilfred Raymond Hagen; Hans Erik Mølager Christensen
Journal:  J Biol Inorg Chem       Date:  2013-01-08       Impact factor: 3.358

3.  Ferredoxin III of Desulfovibrio africanus: sequencing of the native gene and characterization of a histidine-tagged form.

Authors:  J L Busch; J L Breton; S L Davy; R James; G R Moore; F A Armstrong; A J Thomson
Journal:  Biochem J       Date:  2000-03-01       Impact factor: 3.857

4.  Expression in Escherichia coli and characterization of a reconstituted recombinant 7Fe ferredoxin from Desulfovibrio africanus.

Authors:  J L Busch; J L Breton; B M Bartlett; R James; E C Hatchikian; A J Thomson
Journal:  Biochem J       Date:  1996-02-15       Impact factor: 3.857

5.  Voltammetric studies of the reactions of iron-sulphur clusters ([3Fe-4S] or [M3Fe-4S]) formed in Pyrococcus furiosus ferredoxin.

Authors:  S E Fawcett; D Davis; J L Breton; A J Thomson; F A Armstrong
Journal:  Biochem J       Date:  1998-10-15       Impact factor: 3.857

6.  The iron-sulfur clusters of dehydratases are primary intracellular targets of copper toxicity.

Authors:  Lee Macomber; James A Imlay
Journal:  Proc Natl Acad Sci U S A       Date:  2009-05-04       Impact factor: 11.205
  6 in total

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