Literature DB >> 76622

An improved Timm sulphide silver method for light and electron microscopic localization of heavy metals in biological tissues.

G Danscher, J Zimmer.   

Abstract

Modifications of the Timm sulphide silver method for the demonstration of heavy metals are described. To improve the structural preservation of the tissues perfusion with a glutaraldehyde fixature is employed before perfusion with the sodium sulphide solution. For the subsequent staining for light and electron microscopy, procedures for plastic embedding, paraffin embedding and cryostat sectioning are presented. Examples from several tissues are shown, including the pituitary, pancreas, intestine, tongue, kidney, testis and brain. The staining of autolytic, postmortal human brain tissue is demonstrated.

Entities:  

Mesh：

Substances：

Year:  1978        PMID： 76622     DOI： 10.1007/BF00496691

Source DB:  PubMed          Journal:  Histochemistry        ISSN： 0301-5564


  37 in total

1.  Persistent function of mossy fibre synapses after metal chelation with DEDTC (Antabuse).

Authors:  G Danscher; M T Shipley; P Andersen
Journal:  Brain Res       Date:  1975-03-07       Impact factor: 3.252

2.  Effect of intravital dithizone treatment on the Timm sulfide silver pattern of rat brain.

Authors:  F M Haug; G Danscher
Journal:  Histochemie       Date:  1971

3.  Electron microscopical localization of the zinc in hippocampal mossy fibre synapses by a modified sulfide silver procedure.

Authors:  F M Haug
Journal:  Histochemie       Date:  1967

4.  The histochemical architecture of the Ammon's horn as related to its selective vulnerability.

Authors:  R L Friede
Journal:  Acta Neuropathol       Date:  1966-01-14       Impact factor: 17.088

5.  [An electron microscopic test for heavy metals in the prosecretion granules in Paneth's cells].

Authors:  A Müller; G Geyer
Journal:  Acta Histochem       Date:  1965-08-14       Impact factor: 2.479

6.  Sulfide silver architectonics of rat, cat, and guinea pig spinal cord. A light microscopic study with Timm's method for demonstration of heavy metals.

Authors:  H D Schroder
Journal:  Anat Embryol (Berl)       Date:  1977-05-12

7.  [Histochemical and radioautographical studies on the hippocampus formation of the mouse].

Authors:  N Otsuka; M Kawamoto
Journal:  Histochemie       Date:  1966

8.  Heavy metals in the brain. A light microscope study of the rat with Timm's sulphide silver method. Methodological considerations and cytological and regional staining patterns.

Authors:  F M Smejda Haug
Journal:  Adv Anat Embryol Cell Biol       Date:  1973       Impact factor: 1.231

9.  [CARNOY SODIUM SULFIDE MIXTURE AS A FIXATIVE AGENT IN THE TIMM SULFUR-SILVER METHOD].

Authors:  R KODOUSEK
Journal:  Acta Histochem       Date:  1963-06-30       Impact factor: 2.479

10.  Improvements in epoxy resin embedding methods.

Authors:  J H LUFT
Journal:  J Biophys Biochem Cytol       Date:  1961-02
View more
  50 in total

1.  Timm-staining intensity is correlated with the density of Timm-positive presynaptic structures in the cerebral cortex of lizards.

Authors:  F J Martinez-Guijarro; A Molowny; C Lopez-Garcia
Journal:  Histochemistry       Date:  1987

2.  Hippocampal morphology in the inbred mouse strains NZB and CBA/H and their reciprocal congenics for the nonpseudoautosomal region of the Y chromosome.

Authors:  P V Guillot; F Sluyter; A Laghmouch; P L Roubertoux; W E Crusio
Journal:  Behav Genet       Date:  1996-01       Impact factor: 2.805

3.  New silver-gold intensification method of diaminobenzidine for double-labeling immunoelectron microscopy.

Authors:  Endre Dobó; Virág T Takács; Attila I Gulyás; Gábor Nyiri; András Mihály; Tamás F Freund
Journal:  J Histochem Cytochem       Date:  2011-01-12       Impact factor: 2.479

4.  Elimination of zinc from synaptic vesicles in the intact mouse brain by disruption of the ZnT3 gene.

Authors:  T B Cole; H J Wenzel; K E Kafer; P A Schwartzkroin; R D Palmiter
Journal:  Proc Natl Acad Sci U S A       Date:  1999-02-16       Impact factor: 11.205

5.  The autometallographic zinc-sulphide method. A new approach involving in vivo creation of nanometer-sized zinc sulphide crystal lattices in zinc-enriched synaptic and secretory vesicles.

Authors:  G Danscher
Journal:  Histochem J       Date:  1996-05

6.  Selective loss of hilar neurons and impairment of initial learning in rats after repeated administration of electroconvulsive shock seizures.

Authors:  Nikolai V Lukoyanov; Maria J Sá; M Dulce Madeira; Manuel M Paula-Barbosa
Journal:  Exp Brain Res       Date:  2003-10-14       Impact factor: 1.972

7.  Cadmium kinetics in freshwater clams. IV. Histochemical localization of cadmium in Anodonta cygnea and Anodonta anatina, exposed to cadmium chloride.

Authors:  J Hemelraad; H J Herwig
Journal:  Arch Environ Contam Toxicol       Date:  1988-05       Impact factor: 2.804

8.  Alloxan cytotoxicity is highly potentiated by plasma membrane- and lysosomal-associated iron--a study on a model system of cultured J-774 cells.

Authors:  H Zhang; U T Brunk
Journal:  Diabetologia       Date:  1993-08       Impact factor: 10.122

9.  Sexual dimorphism in the mossy fiber synapses of the rat hippocampus.

Authors:  M D Madeira; N Sousa; M M Paula-Barbosa
Journal:  Exp Brain Res       Date:  1991       Impact factor: 1.972

10.  Heavy metals in the spinal cord of normal rats and of animals treated with chelating agents: a quantitative (zinc, copper, and lead) and histochemical study.

Authors:  H D Schrøder; E Fjerdingstad; G Danscher; E J Fjerdingstad
Journal:  Histochemistry       Date:  1978-06-02
View more

北京卡尤迪生物科技股份有限公司 © 2022-2023.