PURPOSE: Cytokines have been implicated in corneal inflammatory diseases. The authors sought to determine if corneal diseases with different patterns of inflammation are accompanied by characteristic patterns of cytokine expression. METHODS: The authors used reverse transcription-polymerase chain reaction to detect mRNA for 12 specific cytokines from patients with Fuch's dystrophy (n = 7), pseudophakic bullous keratopathy (n = 12), or normal corneas (n = 4). RESULTS: Using Wilcoxon rank sum analysis, mRNA for interleukin (IL)-1 alpha or IL-8 was significantly more abundant in corneas from patients with pseudophakic bullous keratopathy relative to either comparison group. mRNA for the T-cell marker, CD4, and for T-cell derived cytokines, IL-2, IL-4, and interferon gamma, could not be detected in any corneal sample. Message for IL-1 receptor antagonist and transforming growth factor-beta 1 or -beta 2 was readily detectable in most corneas regardless of diagnosis. CONCLUSION: The findings indicate that the pattern of cytokine mRNA expression reflects differences in the pathogenesis of these corneal diseases.
PURPOSE: Cytokines have been implicated in corneal inflammatory diseases. The authors sought to determine if corneal diseases with different patterns of inflammation are accompanied by characteristic patterns of cytokine expression. METHODS: The authors used reverse transcription-polymerase chain reaction to detect mRNA for 12 specific cytokines from patients with Fuch's dystrophy (n = 7), pseudophakic bullous keratopathy (n = 12), or normal corneas (n = 4). RESULTS: Using Wilcoxon rank sum analysis, mRNA for interleukin (IL)-1 alpha or IL-8 was significantly more abundant in corneas from patients with pseudophakic bullous keratopathy relative to either comparison group. mRNA for the T-cell marker, CD4, and for T-cell derived cytokines, IL-2, IL-4, and interferon gamma, could not be detected in any corneal sample. Message for IL-1 receptor antagonist and transforming growth factor-beta 1 or -beta 2 was readily detectable in most corneas regardless of diagnosis. CONCLUSION: The findings indicate that the pattern of cytokine mRNA expression reflects differences in the pathogenesis of these corneal diseases.
Authors: Zahra Sadrai; Amir Reza Hajrasouliha; Sunil Chauhan; Daniel R Saban; Mohammad H Dastjerdi; Reza Dana Journal: Invest Ophthalmol Vis Sci Date: 2011-04-19 Impact factor: 4.799
Authors: Ying-Ting Chen; Stanislav Lazarev; Ahmad F Bahrami; Lisa B Noble; Feeling Y T Chen; Delu Zhou; Marianne Gallup; Mahesh Yadav; Nancy A McNamara Journal: Lab Invest Date: 2012-01-09 Impact factor: 5.662
Authors: Francisco Amparo; Mohammad H Dastjerdi; Andre Okanobo; Giulio Ferrari; Leila Smaga; Pedram Hamrah; Ula Jurkunas; Debra A Schaumberg; Reza Dana Journal: JAMA Ophthalmol Date: 2013-06 Impact factor: 7.389