Literature DB >> 765743

The organization of genes in yeast mitochondrial DNA II. The physical map of EcoRI and HindII + III fragments.

J P Sanders, P Borst, P J Weijers.   

Abstract

1. We have isolated large fragments of the mtDNA of the yeast Saccharomyces carlsbergensis and digested these with restriction endonucleases. The digestion products were separated by electrophoresis in agarose gels. 2. Endonucleases EcoRI, HindII + III, HpaI, HindIII and HapII yield 9, 11, 6, 0 and greater than 80 fragments, respectively. 3. By analysis of partial digestion products and by redigesting the fragments obtained with one endonuclease with a second, we have established the order of all EcoRI and HindII + III fragments. The map is circular and its contour length is 22.1 +/- 0.35 mum, in good agreement with earlier estimates of the size of yeast mtDNA, using electron microscopy and renaturation kinetics. 4. A comparison of the fragmentation pattern of mtDNAs from S. carlsbergensis and various strains of Saccharomyces cerevisiae with endonuclease HindII + III suggests that the overall gene order is similar.

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Year:  1975        PMID： 765743     DOI： 10.1007/bf00269420

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  41 in total

1.  A new method for isolation of a restriction enzyme from Hemophilus parainfluenzae.

Authors:  F M DeFilippes
Journal:  Biochem Biophys Res Commun       Date:  1974-06-04       Impact factor: 3.575

2.  Endonuclease R. Hind fragments of T7 DNA.

Authors:  P Humphries; R L Gordon; D J McConnell; P Connolly
Journal:  Virology       Date:  1974-03       Impact factor: 3.616

3.  Mitochondrial nucleic acids in the petite colonie mutants: deletions and repetition of genes.

Authors:  G Faye; H Fukuhara; C Grandchamp; J Lazowska; F Michel; J Casey; G S Getz; J Locker; M Rabinowitz; M Bolotin-Fukuhara; D Coen; J Deutsch; B Dujon; P Netter; P P Slonimski
Journal:  Biochimie       Date:  1973       Impact factor: 4.079

4.  The mitochondrial genome of wild-type yeast cells. II. Investigations on the compositional heterogeneity of mitochondrial DNA.

Authors:  G Piperno; G Fonty; G Bernardi
Journal:  J Mol Biol       Date:  1972-03-28       Impact factor: 5.469

5.  Nucleotide sequences at the cleavage sites of two restriction endonucleases from Hemophilus parainfluenzae.

Authors:  D E Garfin; H M Goodman
Journal:  Biochem Biophys Res Commun       Date:  1974-07-10       Impact factor: 3.575

6.  Polyoma DNA: a physical map.

Authors:  B E Griffin; M Fried; A Cowie
Journal:  Proc Natl Acad Sci U S A       Date:  1974-05       Impact factor: 11.205

7.  Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis.

Authors:  P A Sharp; B Sugden; J Sambrook
Journal:  Biochemistry       Date:  1973-07-31       Impact factor: 3.162

8.  Role of molecular conformation in determining the electrophoretic properties of polynucleotides in agarose-acrylamide gels. II.

Authors:  C W Dingman; M P Fisher; T Kakefuda
Journal:  Biochemistry       Date:  1972-03-28       Impact factor: 3.162

9.  Analysis of endonuclease R-EcoRI fragments of DNA from lambdoid bacteriophages and other viruses by agarose-gel electrophoresis.

Authors:  R B Helling; H M Goodman; H W Boyer
Journal:  J Virol       Date:  1974-11       Impact factor: 5.103

10.  Properties of mitochondrial DNA from Kluyveromyces lactis.

Authors:  J P Sanders; P J Weijers; G S Groot; P Borst
Journal:  Biochim Biophys Acta       Date:  1974-12-06
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  16 in total

1.  Assembly of the mitochondrial membrane system: partial sequence of a mitochondrial ATPase gene in Saccharomyces cerevisiae.

Authors:  G Macino; A Tzagoloff
Journal:  Proc Natl Acad Sci U S A       Date:  1979-01       Impact factor: 11.205

2.  Electrophoretic strand separation of long DNAs with poly (U,G) in agarose gels.

Authors:  R W Goldbach; R F Evers; P Borst
Journal:  Nucleic Acids Res       Date:  1978-08       Impact factor: 16.971

3.  Fine structure physical mapping of 4S RNA genes on mitochondrial DNA of Saccharomyces cerevisiae.

Authors:  G J Van Ommen; G S Groot; P Borst
Journal:  Mol Gen Genet       Date:  1977-09-09

4.  Segments of mitochondrial DNA of yeast carrying antibiotic resistances.

Authors:  K Wakabayashi
Journal:  Mol Gen Genet       Date:  1978-02-27

5.  On the operon structure of the nitrogenase genes of Rhizobium leguminosarum and Azotobacter vinelandii.

Authors:  A J Krol; J G Hontelez; B Roozendaal; A van Kammen
Journal:  Nucleic Acids Res       Date:  1982-07-24       Impact factor: 16.971

6.  The presence of (dA.dT)20-25 tracts in the DNA of primitive eukaryotes.

Authors:  J N Mol; R A Flavell; P Borst
Journal:  Nucleic Acids Res       Date:  1976-09       Impact factor: 16.971

7.  Physical mapping of the Xba I, Hinc II, Bgl II, Xho I, Sst I, and Pvu II restriction endonuclease cleavage fragments of mitochondrial DNA of S. cerevisiae.

Authors:  R Morimoto; M Rabinowitz
Journal:  Mol Gen Genet       Date:  1979-02-16

8.  Physical mapping of the yeast mitochondrial genome: derivation of the fine structure and gene map of strain D273-10B and comparison with a strain (MH41-7B) differing in genome size.

Authors:  R Morimoto; M Rabinowitz
Journal:  Mol Gen Genet       Date:  1979-02-16

9.  Detection of specific DNA sequences in yeast by colony hybridization.

Authors:  H Blanc; B Dujon; M Guerineau; P P Slonimski
Journal:  Mol Gen Genet       Date:  1978-05-31

10.  In vitro splicing of the terminal intervening sequence of Saccharomyces cerevisiae cytochrome b pre-mRNA.

Authors:  A Gampel; A Tzagoloff
Journal:  Mol Cell Biol       Date:  1987-07       Impact factor: 4.272
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