| Literature DB >> 7655624 |
B O Axelsson1, A Saraf, L Larsson.
Abstract
A gas chromatographic-mass spectrometric method was developed for the determination of ergosterol in organic dust. Samples were hydrolyzed under alkaline conditions, and the hydrolysate was extracted, purified on a silica-gel column, and subjected to derivatization. The limit of detection of the trimethylsilyl ether derivative of ergosterol was approximately 10 pg and that of the tert.-butyldimethylsilyl ether derivative was approximately 20 pg (injected amounts). House dust contained 6-45 micrograms ergosterol/g and air from a pig barn contained 0.2-0.3 ng ergosterol/liter. The proposed method can be used as a complement or alternative to microscopy and culturing for measuring fungal biomass in air-borne organic dust.Entities:
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Year: 1995 PMID： 7655624 DOI： 10.1016/0378-4347(94)00553-h
Source DB: PubMed Journal: J Chromatogr B Biomed Appl ISSN： 1572-6495