Literature DB >> 7651334

Transposon mutagenesis and cloning of the genes encoding the enzymes of fengycin biosynthesis in Bacillus subtilis.

C L Chen1, L K Chang, Y S Chang, S T Liu, J S Tschen.   

Abstract

A total of 20 Bacillus subtilis F29-3 mutants defective in fengycin biosynthesis was obtained by Tn917 mutagenesis. Cloning and mapping results showed that the transposon in these mutants was inserted in eleven different locations on the chromosome. We were able to use the chromosomal sequence adjacent to the transposon as a probe to screen for cosmid clones containing the fengycin biosynthesis genes. One of the clones obtained, pFC660, was 46 kb long. Eight transposon insertion sites were mapped within this plasmid. Among the eleven different mutants analyzed, four mutants had Tn917 inserted in regions which encoded peptide sequences similar to part of gramicidin S synthetase, surfactin synthetase, and tyrocidine synthetase. Our results suggest that fengycin is synthesized nonribosomally by the multienzyme thiotemplate mechanism.

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Year:  1995        PMID： 7651334     DOI： 10.1007/bf02190792

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  35 in total

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Journal:  J Bacteriol       Date:  1973-06       Impact factor: 3.490

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Journal:  Mol Gen Genet       Date:  1984

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  10 in total

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9.  Water surface tension modulates the swarming mechanics of Bacillus subtilis.

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  10 in total

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