Literature DB >> 7649178

13C-NMR spectroscopy of keratan sulphates. Assignments for four poly(N-acetyllactosamine)-repeat-sequence tetrasaccharides derived from bovine articular cartilage keratan sulphate by keratanase II digestion.

T N Huckerby1, G M Brown, I A Nieduszynski.   

Abstract

Skeletal keratan sulphate has been fragmented using the enzyme keratanase II, and the complete 13C chemical shift data are reported for the four tetrasaccharides which derived from the repeat region. They have the structures: Gal beta(1-4)GlcNAc(6S)beta(1-3)Gal beta(1-4)GlcNAc(6S)-ol, Gal(6S)beta(1-4)GlcNAc(6S)beta(1-3)Gal(1-4)GlcNAc(6S)-ol, Gal beta(1-4)GlcNAc(6S)beta(1-3)Gal(6S)beta(1-4)GlcNAc(6S)-ol, and Gal(6S)beta(1-4)GlcNAc(6S)(1-3)Gal(6S)beta(1-4)GlcNAc(6S)-ol, where GlcNAc(6S)-ol represents N-acetyl-glucosaminitol-6-O-sulphate. The value of these data for microstructural analysis of undegraded keratan sulphate samples using 13C-NMR spectroscopy is discussed with respect to galactose sulphation levels and the block sulphation structure.

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Year:  1995        PMID： 7649178     DOI： 10.1111/j.1432-1033.1995.0779d.x

Source DB:  PubMed          Journal:  Eur J Biochem        ISSN： 0014-2956


  4 in total

1.  Characterization of a non-reducing terminal fragment from bovine articular cartilage keratan sulphates containing alpha(2-3)-linked sialic acid and alpha(1-3)-linked fucose. A sulphated variant of the VIM-2 epitope.

Authors:  G M Brown; T N Huckerby; B L Abram; I A Nieduszynski
Journal:  Biochem J       Date:  1996-10-01       Impact factor: 3.857

2.  Binding specificity of R-10G and TRA-1-60/81, and substrate specificity of keratanase II studied with chemically synthesized oligosaccharides.

Authors:  Hiromi Nakao; Yuko Nagai; Aya Kojima; Hidenao Toyoda; Nobuko Kawasaki; Toshisuke Kawasaki
Journal:  Glycoconj J       Date:  2017-03-14       Impact factor: 2.916

Review 3.  High-field NMR as a technique for the determination of polysaccharide structures.

Authors:  B Mulloy
Journal:  Mol Biotechnol       Date:  1996-12       Impact factor: 2.695

4.  Proteomic and postproteomic characterization of keratan sulfate-glycanated isoforms of thyroglobulin and transferrin uniquely elaborated by papillary thyroid carcinomas.

Authors:  Gaetano Magro; Daniela Perissinotto; Monica Schiappacassi; Steffen Goletz; Albrecht Otto; Eva-Christina Müller; Michele Bisceglia; Gavin Brown; Timothy Ellis; Sebastiano Grasso; Alfonso Colombatti; Roberto Perris
Journal:  Am J Pathol       Date:  2003-07       Impact factor: 4.307
  4 in total

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