Literature DB >> 7647251

Two-dimensional crystallization of avidin on biotinylated lipid monolayers.

H Qin1, Z Liu, S F Sui.   

Abstract

Two-dimensional crystals of avidin were obtained on mixed lipid monolayers containing biotinylated lipids (N-biotinyl-dipalmitoyl-L-alpha-phosphatidyl ethanolamine and dioleoyl phosphatidyl choline) by specific interaction. Image analysis of electron micrographs of these crystals revealed p2 symmetry with the unit cell parameters a = 66 +/- 2 A, b = 68 +/- 1 A, and gamma = 121 +/- 4 degrees. The projection map showed, at a resolution of about 27 A, that the four subunits within one avidin molecule are separated into two parts. Comparison between avidin and streptavidin reveals that avidin molecule binds to the lipid monolayer in an orientation similar to that of streptavidin.

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Year:  1995        PMID： 7647251      PMCID： PMC1282158          DOI： 10.1016/S0006-3495(95)80431-5

Source DB:  PubMed          Journal:  Biophys J        ISSN： 0006-3495            Impact factor:   4.033


  14 in total

1.  AVIDIN. 2. PURIFICATION AND COMPOSITION.

Authors:  M D MELAMED; N M GREEN
Journal:  Biochem J       Date:  1963-12       Impact factor: 3.857

Review 2.  Introduction to avidin-biotin technology.

Authors:  M Wilchek; E A Bayer
Journal:  Methods Enzymol       Date:  1990       Impact factor: 1.600

Review 3.  The avidin-biotin complex in bioanalytical applications.

Authors:  M Wilchek; E A Bayer
Journal:  Anal Biochem       Date:  1988-05-15       Impact factor: 3.365

4.  Molecular cloning and nucleotide sequence of the streptavidin gene.

Authors:  C E Argaraña; I D Kuntz; S Birken; R Axel; C R Cantor
Journal:  Nucleic Acids Res       Date:  1986-02-25       Impact factor: 16.971

Review 5.  Three-dimensional structure determination by electron microscopy of two-dimensional crystals.

Authors:  L A Amos; R Henderson; P N Unwin
Journal:  Prog Biophys Mol Biol       Date:  1982       Impact factor: 3.667

6.  Compositional and structural heterogeneity of avidin glycopeptides.

Authors:  R C Bruch; H B White
Journal:  Biochemistry       Date:  1982-10-12       Impact factor: 3.162

7.  Model for the structure of bacteriorhodopsin based on high-resolution electron cryo-microscopy.

Authors:  R Henderson; J M Baldwin; T A Ceska; F Zemlin; E Beckmann; K H Downing
Journal:  J Mol Biol       Date:  1990-06-20       Impact factor: 5.469

8.  Two-dimensional crystallization technique for imaging macromolecules, with application to antigen--antibody--complement complexes.

Authors:  E E Uzgiris; R D Kornberg
Journal:  Nature       Date:  1983-01-13       Impact factor: 49.962

9.  On the mode of liposome-cell interactions. Biotin-conjugated lipids as ultrastructural probes.

Authors:  E A Bayer; B Rivnay; E Skutelsky
Journal:  Biochim Biophys Acta       Date:  1979-02-02

10.  Two-dimensional crystals of streptavidin on biotinylated lipid layers and their interactions with biotinylated macromolecules.

Authors:  S A Darst; M Ahlers; P H Meller; E W Kubalek; R Blankenburg; H O Ribi; H Ringsdorf; R D Kornberg
Journal:  Biophys J       Date:  1991-02       Impact factor: 4.033
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  3 in total

1.  The impact of physiological crowding on the diffusivity of membrane bound proteins.

Authors:  Justin R Houser; David J Busch; David R Bell; Brian Li; Pengyu Ren; Jeanne C Stachowiak
Journal:  Soft Matter       Date:  2016-01-11       Impact factor: 3.679

2.  Visualization of synaptotagmin I oligomers assembled onto lipid monolayers.

Authors:  Yi Wu; Yuhong He; Jihong Bai; Shang-Rong Ji; Ward C Tucker; Edwin R Chapman; Sen-Fang Sui
Journal:  Proc Natl Acad Sci U S A       Date:  2003-02-10       Impact factor: 11.205

Review 3.  Biological cryo-electron microscopy in China.

Authors:  Hong-Wei Wang; Jianlin Lei; Yigong Shi
Journal:  Protein Sci       Date:  2016-09-02       Impact factor: 6.725
  3 in total

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