| Literature DB >> 7646472 |
K P Vercruysse1, A R Lauwers, J M Demeester.
Abstract
In this paper we describe kinetic investigations of the action of testicular hyaluronidase on hyaluronan. We have compared the use of two spectrophotometric assays, the first based on the Morgan-Elson reaction and the second on the neocuproine reaction. With the neocuproine reaction Km was found to be 0.46 mg/ml and Vmax to be 126 nmol l-1 s-1. Because of a low sensitivity and the production of interfering precipitates, the Morgan-Elson assay cannot be used for kinetic investigation of the enzyme. Furthermore this assay is prone to interference from compounds such as disodium cromoglycate, (+)-catechine, penicillamine, CaCl2 and acetate buffer.Entities:
Mesh：
Substances：
Year: 1995 PMID： 7646472 PMCID： PMC1135853 DOI： 10.1042/bj3100055
Source DB: PubMed Journal: Biochem J ISSN： 0264-6021 Impact factor: 3.857