Coat protein gene sequence of an Austrian isolate of grapevine fanleaf virus.

The nucleotide sequence of the coat protein cistron of an Austrian isolate of grapevine fanleaf virus (GFLV-FC) from Vitis vinifera cv. French Colombard was determined. It shows small differences at the RNA level as well as at the protein level compared to the sequences of already published grapevine fanleaf virus strains. The differences may be a result of the natural variation among virus populations or a consequence of selection in a special host plant. As the virus RNA sequence reported here was isolated directly from its natural woody host by an immunocapture-PCR technique, passage of the virus through a herbaceous host could be avoided and a possible bias introduced by a different host environment was excluded. The sequence similarity of known GFLV coat protein cistrons to the sequence described is as high as among the other strains.