Literature DB >> 7646015

Purification and characterization of an extracellular alpha-amylase from Clostridium perfringens type A.

N J Shih1, R G Labbé.   

Abstract

An alpha-amylase (EC 3.2.1.1) secreted by Clostridium perfringens NCTC 8679 type A was purified to homogeneity and characterized. It was isolated from concentrated cell-free culture medium by ion-exchange and gel permeation chromatography. The enzyme exhibited maximal activity at pH 6.5 and 30 degrees C without the presence of calcium. The pI of the enzyme was 4.75. The estimated molecular weight of the purified enzyme was 76 kDa. The purified enzyme was inactivated between 35 and 40 degrees C, which increased to between 45 and 50 degrees C in the presence of calcium (5 mM). The purified enzyme produced a mixture of oligosaccharides as major end products of starch hydrolysis, indicating alpha-amylase activity.

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Year:  1995        PMID： 7646015      PMCID： PMC167440          DOI： 10.1128/aem.61.5.1776-1779.1995

Source DB:  PubMed          Journal:  Appl Environ Microbiol        ISSN： 0099-2240            Impact factor:   4.792


  14 in total

1.  Influence of elevated temperature on starch hydrolysis by enterotoxin-positive and enterotoxin-negative strains of Clostridium perfringens type A.

Authors:  J S García-Alvarado; M A Rodriguez; R G Labbé
Journal:  Appl Environ Microbiol       Date:  1992-01       Impact factor: 4.792

2.  Isolation and characterization of Clostridium acetobutylicum mutants with enhanced amylolytic activity.

Authors:  B A Annous; H P Blaschek
Journal:  Appl Environ Microbiol       Date:  1991-09       Impact factor: 4.792

3.  Influence of carbohydrates on growth and sporulation of Clostridium perfringens type A.

Authors:  R G Labbe; C L Duncan
Journal:  Appl Microbiol       Date:  1975-03

4.  Purification and Properties of a Thermoactive Glucoamylase from Clostridium thermosaccharolyticum.

Authors:  U Specka; F Mayer; G Antranikian
Journal:  Appl Environ Microbiol       Date:  1991-08       Impact factor: 4.792

5.  Production of Thermostable alpha-Amylase, Pullulanase, and alpha-Glucosidase in Continuous Culture by a New Clostridium Isolate.

Authors:  G Antranikian; C Herzberg; G Gottschalk
Journal:  Appl Environ Microbiol       Date:  1987-07       Impact factor: 4.792

6.  Clear, defined medium for the sporulation of Clostridium perfringens.

Authors:  L E Sacks; P A Thompson
Journal:  Appl Environ Microbiol       Date:  1978-02       Impact factor: 4.792

7.  Proteolysis of Clostridium perfringens type A enterotoxin during purification.

Authors:  K B Park; R G Labbé
Journal:  Infect Immun       Date:  1990-06       Impact factor: 3.441

8.  Purification and characterization of the extracellular alpha-amylase from Clostridium acetobutylicum ATCC 824.

Authors:  V Paquet; C Croux; G Goma; P Soucaille
Journal:  Appl Environ Microbiol       Date:  1991-01       Impact factor: 4.792

Review 9.  Microbial amylolytic enzymes.

Authors:  M Vihinen; P Mäntsälä
Journal:  Crit Rev Biochem Mol Biol       Date:  1989       Impact factor: 8.250

10.  Influence of starch source on sporulation and enterotoxin production by Clostridium perfringens type A.

Authors:  R Labbe; E Somers; C Duncan
Journal:  Appl Environ Microbiol       Date:  1976-03       Impact factor: 4.792
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  2 in total

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Authors:  Soni Tiwari; Neha Shukla; Pooja Mishra; Rajeeva Gaur
Journal:  ScientificWorldJournal       Date:  2014-10-27

Review 2.  Marine Microbiological Enzymes: Studies with Multiple Strategies and Prospects.

Authors:  Yan Wang; Qinghao Song; Xiao-Hua Zhang
Journal:  Mar Drugs       Date:  2016-09-22       Impact factor: 5.118
  2 in total

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