Literature DB >> 7645241

A defined system for in vitro lambda DNA packaging.

Y Hwang1, M Feiss.   

Abstract

We constructed a defined in vitro system for packaging lambda DNA which is composed of purified proheads, the noncapsid proteins terminase and gpFI, and the Escherichia coli DNA binding/bending protein IHF. The defined packaging system: (i) is free from endogenous ATP, DNAs, and DNases and (ii) packages 30% of the input mature lambda DNA efficiently. In this defined packaging system, IHF and gpFI make modest contributions to packaging efficiency. The defined packaging reactions showed that DNA packaging gave a linear response to the concentration of mature lambda DNA and terminase. DNA packaging showed a sigmoidal relationship with respect to the concentration of ATP and proheads.

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Year:  1995        PMID： 7645241     DOI： 10.1006/viro.1995.1419

Source DB:  PubMed          Journal:  Virology        ISSN： 0042-6822            Impact factor:   3.616


  8 in total

1.  Measurements of single DNA molecule packaging dynamics in bacteriophage lambda reveal high forces, high motor processivity, and capsid transformations.

Authors:  Derek N Fuller; Dorian M Raymer; John Peter Rickgauer; Rae M Robertson; Carlos E Catalano; Dwight L Anderson; Shelley Grimes; Douglas E Smith
Journal:  J Mol Biol       Date:  2007-09-11       Impact factor: 5.469

2.  The DNA maturation domain of gpA, the DNA packaging motor protein of bacteriophage lambda, contains an ATPase site associated with endonuclease activity.

Authors:  Marcos E Ortega; Hélène Gaussier; Carlos E Catalano
Journal:  J Mol Biol       Date:  2007-08-14       Impact factor: 5.469

3.  The effect of N- or C-terminal alterations of the connector of bacteriophage phi29 DNA packaging motor on procapsid assembly, pRNA binding, and DNA packaging.

Authors:  Ying Cai; Feng Xiao; Peixuan Guo
Journal:  Nanomedicine       Date:  2008-01-16       Impact factor: 5.307

4.  Mutations in Nu1, the gene encoding the small subunit of bacteriophage lambda terminase, suppress the postcleavage DNA packaging defect of cosB mutations.

Authors:  Z H Cai; Y Hwang; D Cue; C Catalano; M Feiss
Journal:  J Bacteriol       Date:  1997-04       Impact factor: 3.490

5.  Structural and biochemical characterization of phage λ FI protein (gpFI) reveals a novel mechanism of DNA packaging chaperone activity.

Authors:  Ana Popovic; Bin Wu; Cheryl H Arrowsmith; Aled M Edwards; Alan R Davidson; Karen L Maxwell
Journal:  J Biol Chem       Date:  2012-07-16       Impact factor: 5.157

6.  Defining cosQ, the site required for termination of bacteriophage lambda DNA packaging.

Authors:  D J Wieczorek; M Feiss
Journal:  Genetics       Date:  2001-06       Impact factor: 4.562

7.  Structural basis of DNA packaging by a ring-type ATPase from an archetypal viral system.

Authors:  Herman K H Fung; Shelley Grimes; Alexis Huet; Robert L Duda; Maria Chechik; Joseph Gault; Carol V Robinson; Roger W Hendrix; Paul J Jardine; James F Conway; Christoph G Baumann; Alfred A Antson
Journal:  Nucleic Acids Res       Date:  2022-08-10       Impact factor: 19.160

Review 8.  Viral nanomotors for packaging of dsDNA and dsRNA.

Authors:  Peixuan Guo; Tae Jin Lee
Journal:  Mol Microbiol       Date:  2007-05       Impact factor: 3.501
  8 in total

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