Literature DB >> 7632806

Strategies for the synthesis and screening of glycoconjugates. 2. Covalent immobilization for flow cytometry.

D Vetter1, E M Tate, M A Gallop.   

Abstract

Glycosylamines are readily available carbohydrate derivatives that undergo acylation reactions with homobifunctional N-hydroxysuccinimidyl esters. The product glycosylamides carry a spacer group equipped with one active ester functionality. This route provides well-defined glycoconjugates, which may be cross-linked to various amino-functionalized resins. Carbohydrate recognition of the resulting sugar-bead conjugates is probed by lectin immunostaining or flow cytometry using a fluorescently labeled lectin.

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Year:  1995        PMID: 7632806     DOI: 10.1021/bc00033a014

Source DB:  PubMed          Journal:  Bioconjug Chem        ISSN: 1043-1802            Impact factor:   4.774


  3 in total

Review 1.  Flow cytometry and cell sorting of heterogeneous microbial populations: the importance of single-cell analyses.

Authors:  H M Davey; D B Kell
Journal:  Microbiol Rev       Date:  1996-12

2.  Polyvalent binding to carbohydrates immobilized on an insoluble resin.

Authors:  R Liang; J Loebach; N Horan; M Ge; C Thompson; L Yan; D Kahne
Journal:  Proc Natl Acad Sci U S A       Date:  1997-09-30       Impact factor: 11.205

3.  Chemical glycosylation of cytochrome c improves physical and chemical protein stability.

Authors:  Yamixa Delgado; Moraima Morales-Cruz; José Hernández-Román; Yashira Martínez; Kai Griebenow
Journal:  BMC Biochem       Date:  2014-08-06       Impact factor: 4.059

  3 in total

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