Literature DB >> 7632392

Construction and heterologous expression of a synthetic copy of the cutinase cDNA from Fusarium solani pisi.

I A van Gemeren1, W Musters, C A van den Hondel, C T Verrips.   

Abstract

A copy of the cutinase cDNA from Fusarium solani pisi was constructed starting from synthetic oligonucleotides. For this construction three separate cassettes were made, which were subsequently assembled to form the cutinase gene. Heterologous expression of the synthetic cutinase gene and the subsequent secretion of the recombinant enzyme was achieved in Saccharomyces cerevisiae and Aspergillus awamori.

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Year:  1995        PMID： 7632392     DOI： 10.1016/0168-1656(95)00041-n

Source DB:  PubMed          Journal:  J Biotechnol        ISSN： 0168-1656            Impact factor:   3.307


  6 in total

1.  Impaired secretion of a hydrophobic cutinase by Saccharomyces cerevisiae correlates with an increased association with immunoglobulin heavy-chain binding protein (BiP).

Authors:  C M Sagt; W H Müller; J Boonstra; A J Verkleij; C T Verrips
Journal:  Appl Environ Microbiol       Date:  1998-01       Impact factor: 4.792

2.  Evaluation and application of constitutive promoters for cutinase production by Saccharomyces cerevisiae.

Authors:  Juan Zhang; Yanqiu Cai; Guocheng Du; Jian Chen; Miao Wang; Zhen Kang
Journal:  J Microbiol       Date:  2017-06-30       Impact factor: 3.422

3.  Introduction of an N-glycosylation site increases secretion of heterologous proteins in yeasts.

Authors:  C M Sagt; B Kleizen; R Verwaal; M D de Jong; W H Müller; A Smits; C Visser; J Boonstra; A J Verkleij; C T Verrips
Journal:  Appl Environ Microbiol       Date:  2000-11       Impact factor: 4.792

4.  Impaired cutinase secretion in Saccharomyces cerevisiae induces irregular endoplasmic reticulum (ER) membrane proliferation, oxidative stress, and ER-associated degradation.

Authors:  C M J Sagt; W H Müller; L van der Heide; J Boonstra; A J Verkleij; C T Verrips
Journal:  Appl Environ Microbiol       Date:  2002-05       Impact factor: 4.792

5.  Expression and secretion of defined cutinase variants by Aspergillus awamori.

Authors:  I A van Gemeren; A Beijersbergen; C A van den Hondel; C T Verrips
Journal:  Appl Environ Microbiol       Date:  1998-08       Impact factor: 4.792

6.  A permeable cuticle is associated with the release of reactive oxygen species and induction of innate immunity.

Authors:  Floriane L'Haridon; Angélique Besson-Bard; Matteo Binda; Mario Serrano; Eliane Abou-Mansour; Francine Balet; Henk-Jan Schoonbeek; Stephane Hess; Ricardo Mir; José Léon; Olivier Lamotte; Jean-Pierre Métraux
Journal:  PLoS Pathog       Date:  2011-07-28       Impact factor: 6.823
  6 in total

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