Literature DB >> 7629279

Production of a new monoclonal antibody specific to human des-gamma-carboxyprothrombin in the presence of calcium ions. Application to the development of a sensitive ELISA-test.

M Belle1, R Brebant, R Guinet, M Leclercq.   

Abstract

In order to explore pathologies possibly associated with vitamin K deficiency, several monoclonal antibodies (mAbs) were produced against human Desgamma-Carboxy-Prothrombin (DCP). One of these mAbs, designated C4B6, detected DCP forms in the presence of Calcium ions, confirmed by comparison with the patterns of two electrophoretic techniques: Affino-Immuno-Electrophoresis (CAIE) and Polyacrylamide Gel Electrophoresis followed by Electro-blotting (PAGE-Blot). An Enzyme-Linked-Imunosorbent Assay (ELISA) using mAb C4B6 has been developed, optimized and standardized. It has proven to be specific for DCP forms and has a minimum sensitivity of 0.156 A.U/ml.

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Year:  1995        PMID： 7629279     DOI： 10.1080/15321819508013559

Source DB:  PubMed          Journal:  J Immunoassay        ISSN： 0197-1522


  7 in total

Review 1.  Vitamin K supplementation for cystic fibrosis.

Authors:  Vanitha A Jagannath; Zbys Fedorowicz; Vidhu Thaker; Anne B Chang
Journal:  Cochrane Database Syst Rev       Date:  2015-01-18

Review 2.  New insights into the coagulopathy of liver disease and liver transplantation.

Authors:  M Senzolo; P Burra; E Cholongitas; A-K Burroughs
Journal:  World J Gastroenterol       Date:  2006-12-28       Impact factor: 5.742

3.  Vitamin K supplementation for cystic fibrosis.

Authors:  Vanitha A Jagannath; Vidhu Thaker; Anne B Chang; Amy I Price
Journal:  Cochrane Database Syst Rev       Date:  2020-06-04

Review 4.  Vitamin K supplementation for cystic fibrosis.

Authors:  Vanitha A Jagannath; Vidhu Thaker; Anne B Chang; Amy I Price
Journal:  Cochrane Database Syst Rev       Date:  2017-08-22

5.  Intestinal absorption of mixed micellar phylloquinone (vitamin K1) is unreliable in infants with conjugated hyperbilirubinaemia: implications for oral prophylaxis of vitamin K deficiency bleeding.

Authors:  S P Pereira; M J Shearer; R Williams; G Mieli-Vergani
Journal:  Arch Dis Child Fetal Neonatal Ed       Date:  2003-03       Impact factor: 5.747

6.  VItamin K In PEritonial DIAlysis (VIKIPEDIA): Rationale and study protocol for a randomized controlled trial.

Authors:  Stefanos Roumeliotis; Athanasios Roumeliotis; Panagiotis I Georgianos; Elias Thodis; Leon J Schurgers; Katarzyna Maresz; Theodoros Eleftheriadis; Evangelia Dounousi; Giovanni Tripepi; Francesca Mallamaci; Vassilios Liakopoulos
Journal:  PLoS One       Date:  2022-08-17       Impact factor: 3.752

7.  Prevalence and Predictors of Functional Vitamin K Insufficiency in Mothers and Newborns in Uganda.

Authors:  Data Santorino; Mark J Siedner; Juliet Mwanga-Amumpaire; Martin J Shearer; Dominic J Harrington; Unni Wariyar
Journal:  Nutrients       Date:  2015-10-16       Impact factor: 5.717
  7 in total

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