| Literature DB >> 7628716 |
J R Kappelman1, M Brady, K Knoche, E Murray, T Schoenfeld, R Williams, N Vesselinova.
Abstract
A new restriction endonuclease (ENase), SgfI, has been isolated from the bacterium Streptomyces sp. SgfI recognizes the 8-bp palindrome 5'-GCGATCGC-3' and cleaves double-stranded DNA after the T in this sequence, producing a two-base 3' overhang compatible with PvuI termini. SgfI is a rare-cutting ENase and should be useful for megabase mapping experiments.Entities:
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Year: 1995 PMID： 7628716 DOI： 10.1016/0378-1119(95)00197-e
Source DB: PubMed Journal: Gene ISSN： 0378-1119 Impact factor: 3.688