Literature DB >> 7623785

Effects of magnesium and iron on lipid peroxidation in cultured hepatocytes.

T Günther1, J Vormann, V Höllriegl.   

Abstract

In primary cultures of rat hepatocytes, the effects of extracellular Mg2+ and Fe on lipid peroxidation (LPO) as measured by means of malondialdehyde (MDA) formation were investigated. Incubation of hepatocytes at decreasing extracellular Mg2+ concentration enhanced LPO, depending on extracellular Fe. About 96% of MDA accumulated in the culture medium. Addition of desferrioxamine prevented LPO. Additionally, the formation of oxygen free radicals was determined by fluorescence reduction of cis-parinaric acid. With this method, an immediate decay of fluorescence was found after addition of Fe2+. Fluorescence reduction was completely prevented by desferrioxamine, indicating the function of extracellular Fe. This mechanism may operate additionally to the increase in intracellular Fe and intracellular formation of oxygen free radicals during Mg deficiency in vivo.

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Year:  1995        PMID： 7623785     DOI： 10.1007/bf00944393

Source DB:  PubMed          Journal:  Mol Cell Biochem        ISSN： 0300-8177            Impact factor:   3.396


  21 in total

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Review 5.  The measurement and mechanism of lipid peroxidation in biological systems.

Authors:  J M Gutteridge; B Halliwell
Journal:  Trends Biochem Sci       Date:  1990-04       Impact factor: 13.807

6.  Hepatic mitochondrial malondialdehyde metabolism in rats with chronic iron overload.

Authors:  R S Britton; R O'Neill; B R Bacon
Journal:  Hepatology       Date:  1990-01       Impact factor: 17.425

7.  Zinc deficiency increases the osmotic fragility of rat erythrocytes.

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9.  Measurement of protein using bicinchoninic acid.

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Journal:  Anal Biochem       Date:  1985-10       Impact factor: 3.365

10.  Maternal and fetal iron accumulation in Zn-deficient and salicylate-treated rats.

Authors:  T Günther; R Gossrau; J Vormann; V Höllriegl; R Graf
Journal:  Biol Trace Elem Res       Date:  1988-12       Impact factor: 3.738
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  2 in total

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2.  Magnesium Sulfate Attenuates Lethality and Oxidative Damage Induced by Different Models of Hypoxia in Mice.

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Journal:  Biomed Res Int       Date:  2020-12-18       Impact factor: 3.411
  2 in total

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