Literature DB >> 762194

Establishment of a rat hepatoma cell line which has ornithine carbamoyltransferase activity and grows continuously in arginine-deprived medium.

A Niwa, K Yamamoto, Y Yasumura.   

Abstract

The epithelial cell line, H4-II-E derived from Reuber hepatoma H35 has no significant activity of ornithine carbamoyltransferase (OCT, EC 2.1.3.3) and is not able to grow in arginine-deprived medium. A multi-step selection procedure is described which selects from HR-II-E populations, cells with OCT activity which can grow in arginine-deficient, ornithine-supplemented media.

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Year:  1979        PMID： 762194     DOI： 10.1002/jcp.1040980119

Source DB:  PubMed          Journal:  J Cell Physiol        ISSN： 0021-9541            Impact factor:   6.384


  5 in total

1.  Amino acid and vitamin requirements in mammalian cultured cells.

Authors:  K Yamamoto; A Niwa
Journal:  Amino Acids       Date:  1993-02       Impact factor: 3.520

Review 2.  Transcriptional regulation of genes for ornithine cycle enzymes.

Authors:  M Takiguchi; M Mori
Journal:  Biochem J       Date:  1995-12-15       Impact factor: 3.857

3.  Control of serum protein production in hepatocyte hybridomas: immortalization and expression of normal hepatocyte genes.

Authors:  J Szpirer; C Szpirer; J C Wanson
Journal:  Proc Natl Acad Sci U S A       Date:  1980-11       Impact factor: 11.205

4.  Regulation of expression of genes for enzymes of the mammalian urea cycle in permanent cell-culture lines of hepatic and non-hepatic origin.

Authors:  D F Haggerty; E B Spector; M Lynch; R Kern; L B Frank; S D Cederbaum
Journal:  Mol Cell Biochem       Date:  1983       Impact factor: 3.396

5.  Continuous culture of Reuber hepatoma cells in serum-free arginine-, glutamine- and tyrosine-deprived chemically defined medium.

Authors:  A Niwa; K Yamamoto; K Sorimachi; Y Yasumura
Journal:  In Vitro       Date:  1980-11
  5 in total

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