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Literature DB >> 762155

Characterization of a UGA-suppressing serine tRNA from Schizosaccharomyces pombe with the help of a new in vitro assay system for eukaryotic suppressor tRNAs.

J Kohli, T Kwong, F Altruda, D Söll, G Wahl.

Abstract

Two different allele-specific suppressor mutants of the fission yeast Schizosaccharomyces pombe produce opal (UGA) suppressor tRNAs. This was shown by the use of a new in vitro assay for eukaryotic nonsense suppression: a wheat germ extract is programmed with rabbit globin mRNAs and the readthrough products are studied. alpha-Globin is elongated upon addition of ochre (UAA) suppressor tRNAs, whereas beta-globin yields a readthrough product with opal suppressor tRNAs. This simple and very sensitive assay allowed the purification of the opal suppressor tRNA from S. pombe strain sup3-e. The pure tRNA can be aminoacylated with serine; thus, we conclude that this suppressor tRNA inserts serine in response to the UGA termination codon of pure rabbit beta-globin mRNA.

Entities: Chemical Gene Species

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Year: 1979 PMID： 762155

Source DB: PubMed Journal: J Biol Chem ISSN： 0021-9258 Impact factor: 5.157

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Cited

26 in total

6. Polyamines enhance the efficiency of tRNA-mediated readthrough of amber and UGA termination codons in a yeast cell-free system.

Authors: M F Tuite; C S McLaughlin
Journal: Curr Genet Date: 1983-11 Impact factor: 3.886

7. Gene conversion in nonsense suppressors of Schizosaccharomyces pombe. I. The influence of the genetic background and of three mutant genes (rad2, mut1 and mut2) on the frequency of the post-meiotic segregation.

Authors: P Munz; U Leupold
Journal: Mol Gen Genet Date: 1979-02-26

8. The genetic fine structure of the complex locus aro3 involved in early aromatic amino acid biosynthesis in Schizosaccharomyces pombe.

Authors: A Strauss
Journal: Mol Gen Genet Date: 1979

Review 9. Informational suppression as a tool for the investigation of gene structure and function.

Authors: R E Glass; V Nene; M G Hunter
Journal: Biochem J Date: 1982-04-01 Impact factor: 3.857

10. Endogenous read-through of a UGA termination codon in a Saccharomyces cerevisiae cell-free system: evidence for involvement of both a mitochondrial and a nuclear tRNA.

Authors: M F Tuite; C S McLaughlin
Journal: Mol Cell Biol Date: 1982-05 Impact factor: 4.272

Characterization of a UGA-suppressing serine tRNA from Schizosaccharomyces pombe with the help of a new in vitro assay system for eukaryotic suppressor tRNAs.

1. Antagonism of Chk1 signaling in the G2 DNA damage checkpoint by dominant alleles of Cdr1.

2. The genetic fine structure of nonsense suppressors in Schizosaccharomyces pombe : I. sup3 and sup9.

3. Putative frameshift suppressors in Schizosaccharomyces pombe.

4. Gene conversion in nonsense suppressors of Schizosaccharomyces pombe : II. Specific marker effects.

5. The genetic fine structure of nonsense suppressors in Schizosaccharomyces pombe : II. sup8 and sup10.

6. Polyamines enhance the efficiency of tRNA-mediated readthrough of amber and UGA termination codons in a yeast cell-free system.

7. Gene conversion in nonsense suppressors of Schizosaccharomyces pombe. I. The influence of the genetic background and of three mutant genes (rad2, mut1 and mut2) on the frequency of the post-meiotic segregation.

8. The genetic fine structure of the complex locus aro3 involved in early aromatic amino acid biosynthesis in Schizosaccharomyces pombe.

Review 9. Informational suppression as a tool for the investigation of gene structure and function.

10. Endogenous read-through of a UGA termination codon in a Saccharomyces cerevisiae cell-free system: evidence for involvement of both a mitochondrial and a nuclear tRNA.