Nuclear calmodulin responds rapidly to calcium influx at the plasmalemma.

We have studied the rate and extent of calcium binding to calmodulin in neuronal cytosol and nucleus during brief calcium influx across the plasmalemma. Rat sensory neurones were whole-cell patch clamped using a pipette containing a fluorescent analogue of calmodulin that reports when it has bound calcium. Cytosolic and nuclear signals were separated using a confocal microscope. Plasmalemmal calcium influx during a one second depolarization that activated L type calcium channels caused large fractions of calmodulin in both the cytosol and nucleus to bind calcium. Thus, contrary to previous predictions, nuclear processes that require the calcium:calmodulin complex will be activated readily by even brief cell stimulation.