Literature DB >> 7620971

Xenopus oocytes as a heterologous expression system for plant proteins.

F L Theodoulou1, A J Miller.   

Abstract

The Xenopus oocyte is a robust and convenient system for the transient expression of many different animal proteins and it has recently been demonstrated that oocytes can also translate, process, and target plant proteins. This expression system can also be used to clone genes, characterize function, and study posttranslational processing of proteins. Here we describe the methodology for the expression of plant proteins, in particular membrane proteins, in Xenopus oocytes.

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Year:  1995        PMID： 7620971     DOI： 10.1007/BF02789106

Source DB:  PubMed          Journal:  Mol Biotechnol        ISSN： 1073-6085            Impact factor:   2.695


  41 in total

1.  The vacuolar protein-targeting signal of yeast carboxypeptidase is functional in oocytes from Xenopus laevis.

Authors:  T Roitsch; L Lehle
Journal:  Eur J Biochem       Date:  1991-01-01

2.  Purification of mRNA directly from crude plant tissues in 15 minutes using magnetic oligo dT microspheres.

Authors:  K S Jakobsen; E Breivold; E Hornes
Journal:  Nucleic Acids Res       Date:  1990-06-25       Impact factor: 16.971

3.  Expression of an inward-rectifying potassium channel by the Arabidopsis KAT1 cDNA.

Authors:  D P Schachtman; J I Schroeder; W J Lucas; J A Anderson; R F Gaber
Journal:  Science       Date:  1992-12-04       Impact factor: 47.728

Review 4.  Poly(A) tail metabolism and function in eucaryotes.

Authors:  A Sachs; E Wahle
Journal:  J Biol Chem       Date:  1993-11-05       Impact factor: 5.157

Review 5.  Endogenous amino acid transport systems and expression of mammalian amino acid transport proteins in Xenopus oocytes.

Authors:  L J Van Winkle
Journal:  Biochim Biophys Acta       Date:  1993-10-29

6.  Differential targeting of glucose transporter isoforms heterologously expressed in Xenopus oocytes.

Authors:  H M Thomas; J Takeda; G W Gould
Journal:  Biochem J       Date:  1993-03-15       Impact factor: 3.857

7.  Two Closely Related Wheat Storage Proteins Follow a Markedly Different Subcellular Route in Xenopus laevis Oocytes.

Authors:  R. Simon; Y. Altschuler; R. Rubin; G. Galili
Journal:  Plant Cell       Date:  1990-09       Impact factor: 11.277

8.  Analysis and cloning of the ethylene-forming enzyme from tomato by functional expression of its mRNA in Xenopus laevis oocytes.

Authors:  P Spanu; D Reinhardt; T Boller
Journal:  EMBO J       Date:  1991-08       Impact factor: 11.598

9.  The vacuolar membrane protein gamma-TIP creates water specific channels in Xenopus oocytes.

Authors:  C Maurel; J Reizer; J I Schroeder; M J Chrispeels
Journal:  EMBO J       Date:  1993-06       Impact factor: 11.598

10.  Antisense oligonucleotide-directed cleavage of mRNA in Xenopus oocytes and eggs.

Authors:  J Shuttleworth; A Colman
Journal:  EMBO J       Date:  1988-02       Impact factor: 11.598
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  2 in total

1.  Uptake Assays in Xenopus laevis Oocytes Using Liquid Chromatography-mass Spectrometry to Detect Transport Activity.

Authors:  Morten Egevang Jørgensen; Christoph Crocoll; Barbara Ann Halkier; Hussam Hassan Nour-Eldin
Journal:  Bio Protoc       Date:  2017-10-20

2.  Voltage-dependent potassium currents expressed in Xenopus laevis oocytes after injection of mRNA isolated from trophozoites of Giardia lamblia (strain Portland-1).

Authors:  Arturo Ponce; Enedina Jimenez-Cardoso; Leticia Eligio-Garcia
Journal:  Physiol Rep       Date:  2013-12-29
  2 in total

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