Literature DB >> 762015

Biosynthesis and cellular distribution of the two superoxide dismutases of Dactylium dendroides.

A R Shatzman, D J Kosman.   

Abstract

The synthesis and subcellular localization of the two superoxide dismutases of Dactylium dendroides were studied in relation to changes in copper and manganese availability. Cultures grew normally at all medium copper concentrations used (10 nM to 1 mM). In the presence of high (10 muM) copper, manganese was poorly absorbed in comparison to the other metals in the medium. However, cells grown at 10 nM copper exhibited a 3.5-fold increase in manganese content, while the concentration of the other metals remained constant. Cultures grown at 10 nM copper or more had 80% Cu/Zn enzyme and 20% mangani enzyme; the former was entirely in the cytosol, and the latter was mitochondrial. Removal of copper from the medium resulted in decreased Cu/Zn superoxide dismutase synthesis with a concomitant increase in the mangani enzyme such that total cellular superoxide dismutase activity remained constant. The mangani enzyme in excess of the 20% was present in the non-mitochondrial fraction. The mitochondria, therefore, show no variability with respect to superoxide dismutase content, whereas the soluble fraction varies from 100 to 13% Cu/Zn superoxide dismutase. Copper-starved cells that were synthesizing predominantly mangani superoxide dismutase could be switched over to mostly Cu/Zn superoxide dismutase synthesis by supplementing the medium with copper during growth. Immunoprecipitation experiments suggest that the decrease in Cu/Zn activity at low copper concentration is a result of decreased synthesis of that protein rather than the production of an inactive apoprotein.

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Year:  1979        PMID: 762015      PMCID: PMC218452          DOI: 10.1128/jb.137.1.313-320.1979

Source DB:  PubMed          Journal:  J Bacteriol        ISSN: 0021-9193            Impact factor:   3.490


  37 in total

1.  HUMAN HEPATOCUPREIN. ISOLATION OF A COPPER PROTEIN FROM THE SUBCELLULAR SOLUBLE FRACTION OF ADULT HUMAN LIVER.

Authors:  H PORTER; M SWEENEY; E M PORTER
Journal:  Arch Biochem Biophys       Date:  1964-05       Impact factor: 4.013

2.  Genetic regulatory mechanisms in the synthesis of proteins.

Authors:  F JACOB; J MONOD
Journal:  J Mol Biol       Date:  1961-06       Impact factor: 5.469

3.  Biochemistry of dystrophic muscle. Mitochondrial succinate-tetrazolium reductase and adenosine triphosphatase.

Authors:  R J PENNINGTON
Journal:  Biochem J       Date:  1961-09       Impact factor: 3.857

4.  The isolation of the coppercontaining protein cerebrocuprein I from normal human brain.

Authors:  H PORTER; S AINSWORTH
Journal:  J Neurochem       Date:  1959-12       Impact factor: 5.372

5.  Cerebrocuprein I. A copper-containing protein isolated from brain.

Authors:  H PORTER; J FOLCH
Journal:  J Neurochem       Date:  1957       Impact factor: 5.372

6.  Diffusion-in-gel methods for immunological analysis.

Authors:  O OUCHTERLONY
Journal:  Prog Allergy       Date:  1958

7.  Reaction of brain copper proteins with sodium diethyldithiocarbamate in normal and in hepatolenticular degeneration.

Authors:  H PORTER; S AINSWORTH
Journal:  Proc Soc Exp Biol Med       Date:  1958-06

8.  [A micro-method of immuno-electrophoresis].

Authors:  J J SCHEIDEGGER
Journal:  Int Arch Allergy Appl Immunol       Date:  1955

9.  Hepatic aldehyde oxidase. I. Purification and properties.

Authors:  K V RAJAGOPALAN; I FRIDOVICH; P HANDLER
Journal:  J Biol Chem       Date:  1962-03       Impact factor: 5.157

10.  Isolation of purified copper protein from horse liver.

Authors:  M S MOHAMED; D M GREENBERG
Journal:  J Gen Physiol       Date:  1954-03       Impact factor: 4.086

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  3 in total

Review 1.  Elemental economy: microbial strategies for optimizing growth in the face of nutrient limitation.

Authors:  Sabeeha S Merchant; John D Helmann
Journal:  Adv Microb Physiol       Date:  2012       Impact factor: 3.517

2.  Cu,Zn superoxide dismutase and copper deprivation and toxicity in Saccharomyces cerevisiae.

Authors:  M A Greco; D I Hrab; W Magner; D J Kosman
Journal:  J Bacteriol       Date:  1990-01       Impact factor: 3.490

3.  Characterization of superoxide dismutase (SOD-1 and SOD-2) activities in inbred mice: evidence for quantitative variability and possible nonallelic SOD-1 polymorphism.

Authors:  J H Bloor; D Holtz; J Kaars; D J Kosman
Journal:  Biochem Genet       Date:  1983-04       Impact factor: 1.890

  3 in total

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