Literature DB >> 7615770

Escherichia coli adherence to HEp-2 cells with prefixed cells.

H M Zepeda-Lopez1, G M Gonzalez-Lugo.   

Abstract

We describe a new method which uses cold absolute methanol-prefixed cells for adherence of enteropathogenic Escherichia coli to HEp-2 cells. We found that a method using bacteria grown in Penassay broth to 10(6) to 10(7) CFU/ml and incubated with prefixed cells for 3 h at 37 degrees C, showed 100% sensitivity and specificity against a method using live cells.

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Year:  1995        PMID： 7615770      PMCID： PMC228182          DOI： 10.1128/jcm.33.5.1414-1417.1995

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  11 in total

1.  Escherichia coli O128 strains from infants with diarrhea commonly show localized adhesion and positivity in the fluorescent-actin staining test but do not hybridize with an enteropathogenic E. coli adherence factor probe.

Authors:  S M Scotland; H R Smith; B Rowe
Journal:  Infect Immun       Date:  1991-04       Impact factor: 3.441

2.  Comparison of two assay methods for patterns of adherence to HEp-2 cells of Escherichia coli from patients with diarrhea.

Authors:  P A Vial; J J Mathewson; H L DuPont; L Guers; M M Levine
Journal:  J Clin Microbiol       Date:  1990-05       Impact factor: 5.948

3.  Adhesion of enteropathogenic Escherichia coli to human intestinal enterocytes and cultured human intestinal mucosa.

Authors:  S Knutton; D R Lloyd; A S McNeish
Journal:  Infect Immun       Date:  1987-01       Impact factor: 3.441

4.  Plasmid-mediated factors conferring diffuse and localized adherence of enteropathogenic Escherichia coli.

Authors:  J P Nataro; I C Scaletsky; J B Kaper; M M Levine; L R Trabulsi
Journal:  Infect Immun       Date:  1985-05       Impact factor: 3.441

5.  Comparison of a modified adherence assay with existing assay methods for identification of enteroaggregative Escherichia coli.

Authors:  K Haider; S M Faruque; M J Albert; S Nahar; P K Neogi; A Hossain
Journal:  J Clin Microbiol       Date:  1992-06       Impact factor: 5.948

6.  Localized, aggregative, and diffuse adherence to HeLa cells, plastic, and human small intestines by Escherichia coli isolated from patients with diarrhea.

Authors:  T Yamamoto; Y Koyama; M Matsumoto; E Sonoda; S Nakayama; M Uchimura; W Paveenkittiporn; K Tamura; T Yokota; P Echeverria
Journal:  J Infect Dis       Date:  1992-12       Impact factor: 5.226

7.  Distinctive patterns of adherence of enteropathogenic Escherichia coli to HeLa cells.

Authors:  I C Scaletsky; M L Silva; L R Trabulsi
Journal:  Infect Immun       Date:  1984-08       Impact factor: 3.441

8.  An ELISA for the detection of localized adherent classic enteropathogenic Escherichia coli serogroups.

Authors:  M J Albert; M Ansaruzzaman; S M Faruque; P K Neogi; K Haider; S Tzipori
Journal:  J Infect Dis       Date:  1991-11       Impact factor: 5.226

9.  Characterization of enteroadherent-aggregative Escherichia coli, a putative agent of diarrheal disease.

Authors:  P A Vial; R Robins-Browne; H Lior; V Prado; J B Kaper; J P Nataro; D Maneval; A Elsayed; M M Levine
Journal:  J Infect Dis       Date:  1988-07       Impact factor: 5.226

10.  Attaching and effacing activities of rabbit and human enteropathogenic Escherichia coli in pig and rabbit intestines.

Authors:  H W Moon; S C Whipp; R A Argenzio; M M Levine; R A Giannella
Journal:  Infect Immun       Date:  1983-09       Impact factor: 3.441
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  3 in total

1.  Laboratory investigation of enteroaggregative Escherichia coli O untypeable:H10 associated with a massive outbreak of gastrointestinal illness.

Authors:  Y Itoh; I Nagano; M Kunishima; T Ezaki
Journal:  J Clin Microbiol       Date:  1997-10       Impact factor: 5.948

2.  Effects of Variety and Postharvest Handling Practices on Microbial Population at Different Stages of the Value Chain of Fresh Tomato (Solanum lycopersicum) in Western Terai of Nepal.

Authors:  Ram B Khadka; Madan Marasini; Ranjana Rawal; Durga M Gautam; Antonio L Acedo
Journal:  Biomed Res Int       Date:  2017-10-12       Impact factor: 3.411

3.  Atypical enteropathogenic Escherichia coli strains form biofilm on abiotic surfaces regardless of their adherence pattern on cultured epithelial cells.

Authors:  Hebert F Culler; Cristiane M Mota; Cecilia M Abe; Waldir P Elias; Marcelo P Sircili; Marcia R Franzolin
Journal:  Biomed Res Int       Date:  2014-05-06       Impact factor: 3.411
  3 in total

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