Literature DB >> 7614563

Expression of a yeast gene can be blocked by insertion of short yeast DNA fragments between a UAS and the TATA box.

O Vincent1, J M Gancedo.   

Abstract

We have constructed a plasmid, pOV10, which facilitates the introduction of putative upstream activating sequences (UAS) or upstream repressing sequences (URS) from yeast genes into plasmids containing CYCl-lacZ fusions. We have observed that the insertion of yeast sequences from 155 to 195 bp between the UAS and the TATA box of a CYCl-lacZ fusion gene can block beta-galactosidase expression. It is suggested that this block is related to the formation of nucleosomes on the DNA.

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Year:  1995        PMID： 7614563     DOI： 10.1007/BF00352109

Source DB:  PubMed          Journal:  Curr Genet        ISSN： 0172-8083            Impact factor:   3.886


  12 in total

1.  Regulatory regions in the yeast FBP1 and PCK1 genes.

Authors:  J J Mercado; J M Gancedo
Journal:  FEBS Lett       Date:  1992-10-19       Impact factor: 4.124

2.  Elevated recombination rates in transcriptionally active DNA.

Authors:  B J Thomas; R Rothstein
Journal:  Cell       Date:  1989-02-24       Impact factor: 41.582

3.  Regions in the promoter of the yeast FBP1 gene implicated in catabolite repression may bind the product of the regulatory gene MIG1.

Authors:  J J Mercado; O Vincent; J M Gancedo
Journal:  FEBS Lett       Date:  1991-10-07       Impact factor: 4.124

4.  Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors.

Authors:  C Yanisch-Perron; J Vieira; J Messing
Journal:  Gene       Date:  1985       Impact factor: 3.688

5.  Regulation of yeast fructose-1,6-bisphosphatase in strains containing multicopy plasmids coding for this enzyme.

Authors:  R de la Guerra; M D Valdés-Hevia; J M Gancedo
Journal:  FEBS Lett       Date:  1988-12-19       Impact factor: 4.124

6.  Trehalose-6-phosphate, a new regulator of yeast glycolysis that inhibits hexokinases.

Authors:  M A Blázquez; R Lagunas; C Gancedo; J M Gancedo
Journal:  FEBS Lett       Date:  1993-08-23       Impact factor: 4.124

7.  Characterization of the gene for fructose-1,6-bisphosphatase from Saccharomyces cerevisiae and Schizosaccharomyces pombe. Sequence, protein homology, and expression during growth on glucose.

Authors:  D T Rogers; E Hiller; L Mitsock; E Orr
Journal:  J Biol Chem       Date:  1988-05-05       Impact factor: 5.157

8.  Fusion of Escherichia coli lacZ to the cytochrome c gene of Saccharomyces cerevisiae.

Authors:  L Guarente; M Ptashne
Journal:  Proc Natl Acad Sci U S A       Date:  1981-04       Impact factor: 11.205

9.  A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.

Authors:  R S Sikorski; P Hieter
Journal:  Genetics       Date:  1989-05       Impact factor: 4.562

10.  A functional role for nucleosomes in the repression of a yeast promoter.

Authors:  C Straka; W Hörz
Journal:  EMBO J       Date:  1991-02       Impact factor: 11.598
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  1 in total

1.  A 27 kDa protein binds to a positive and a negative regulatory sequence in the promoter of the ICL1 gene from Saccharomyces cerevisiae.

Authors:  I Ordiz; P Herrero; R Rodicio; J M Gancedo; F Moreno
Journal:  Biochem J       Date:  1998-01-15       Impact factor: 3.857
  1 in total

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